en Radiobiology Radiobiology تحقيق بديع Original Research <div style="text-align: justify;">Background: Lycium barbarum is a traditional Chinese medicine. Its pharmacological effects mainly rely on a component called Lycium barbarum polysaccharide (LBP). The present study aims to explore the mechanism by which LBP reduces radiation damage in X-ray-irradiated cultured mouse bone marrow mononuclear cells （BMNCs） and the potential involvement of apoptosis. Materials and Methods: Mouse BMNCs were cultured in vitro and exposed to radiation. After 24 hours of LBP treatment, BMNCs viability was detected by cck-8 method, apoptosis rate was examined by Flow cytometry (FCM), Mitochondrial membrane potential (MMP) fluorescence was detected by JC-1, and the expression of mitochondrial pathway-associated protein was measured by immunoblotting. Results: LBP significantly increased BMNCs viability and reduced the apoptosis rate of radiation-exposed BMNCs 24 h after treatment compared to the non-treated control group. In a mitochondrial membrane potential fluorescence assay, LBP reduced the radiation-induced decrease in mitochondrial membrane potential. Western blot analysis further proved this point, where LBP inhibited the release of cytochrome C from mitochondria and also inhibited the expression of caspase 9 and other mitochondrial-related proteins. Conclusions: The mechanism of LBP action in radiation-exposed mouse BMNCs cells seems to involve inhibition of the mitochondrial apoptosis pathway.</div> &nbsp; Lycium barbarum polysaccharide, radiation, apoptosis, in vitro. 1 7 http://ijrr.com/browse.php?a_code=A-10-2188-53&slc_lang=en&sid=1 H. Huang 7900319475328460020676 7900319475328460020676 No Department of Radiology, Fuling Central Hospital of Chongqing City, Chongqing, 408099, China Y. Wang 7900319475328460020677 7900319475328460020677 No Ultrasound diagnostic department, Hubei Maternal and Children Health Hospital, Wuhan, 430070, China L. Zhang 7900319475328460020678 7900319475328460020678 No Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China F.Q. Hu 7900319475328460020679 7900319475328460020679 No Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China J. Li 7900319475328460020680 7900319475328460020680 No Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China H. Pang phua1973@163.com 7900319475328460020681 7900319475328460020681 Yes Department of Nuclear Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China