International Journal of Radiation Research
نشریه پرتو پژوه
Int J Radiat Res
Basic Sciences
http://ijrr.com
79
journal79
2322-3243
2345-4229
10.61186/ijrr
en
jalali
1401
1
1
gregorian
2022
4
1
20
2
online
1
fulltext
en
Cytogenetic and immunological efficacy of nicotiflorin and rutin combination on gamma irradiated rats
Radiation Biology
Radiation Biology
تحقيق بديع
Original Research
<div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:inter-word"><span style="line-height:150%"><span style="font-family:"Times New Roman""><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-GB">Cytogenetic and immunological damages after ionizing radiation exposure are critical factors that lead to many different events and consequences cascade reactions starting from inflammation, ending with cell damage. These biological events if not well controlled will lead to deleterious effects and cancer. The present study aimed to evaluate the efficacy of the Nicotiflorin and Rutin (NR) combination against drastic effects resulted from γ-irradiation.</span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Materials and Methods</span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-GB">: </span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-GB">Rats were divided into four groups. (Control) group, (NR) group: treated with 20mg/kg body weight orally daily one dose for two weeks, (Irrad) group: rats exposed to 6Gy as a single dose, and (NR+ Irrad) group which treated with NR combination before irradiation. Detection of DNA damage was done with the Micronucleus test (MN) and Comet assay. Immunological responses were detected by assessing inflammatory cytokines Interluekines-1β (IL-1β), tumor necrosis factor (TNF-α), and homeostasis maintenance cytokines (IL-6 & IL-10).</span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Results</span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-GB">:</span></span></span></span></span> <span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-GB">Irradiated group recorded a significant increase in micronuclei (MNi) incidences and significant DNA fragmentation. As well, immunological parameters displayed a significant increase in all measured interleukins except IL-10 which recorded a significant decline. The group injected with NR before irradiation showed significant improvement in all measured parameters. </span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-GB">Conclusion</span></span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-GB">: </span></span></span></span></span><span lang="en-GB" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-GB">The efficacy of the NR combination may be attributed to its dual protective effects (cytogenetic and immunological enhancement) against damage caused by γ-irradiation.</span></span></span></span></span></span></span></span></div>
Comet assay, micronucleus, immunomodulation, inflammation, natural products.
455
460
http://ijrr.com/browse.php?a_code=A-10-1-929&slc_lang=en&sid=1
M.R.
Mohammed
7900319475328460021678
7900319475328460021678
No
Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P. O. Box; 29 Nasr City, Cairo, Egypt
M.M.
Ahmed
mahmoud_70mohameds@yahoo.com
7900319475328460021679
7900319475328460021679
Yes
Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P. O. Box; 29 Nasr City, Cairo, Egypt
S.A.
Montaser
7900319475328460021680
7900319475328460021680
No
Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P. O. Box; 29 Nasr City, Cairo, Egypt