International Journal of Radiation Research

en Liposomal silibinin as a potential radioprotector of human lymphocytes in the treatment of non-small cell lung cancer Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: This study aimed to investigate the radioprotective effect of liposomal silibinin (Lip-SIL) on human lymphocytes in the treatment of non-small lung cancer cells using a combined method of cell viability assay and cytokinesis-block micronucleus assay for a better evaluation of whether one active compound is suitable to be used as a radioprotector in radiotherapy or not. Materials and Methods: Firstly, Lip-SIL was prepared by the lipid film hydration method combined with sonication. Secondly, penetration of Lip-SIL into cells was observed by fluorescence microscopy. Finally, the potential application of Lip-SIL as a radioprotector of human lymphocytes in the treatment of non-small cell lung cancer was evaluated using the above combined method with A549 cell line as a model. Results: The successfully prepared Lip-SIL had a spherical shape and good physical characteristics (particle size of approximately 83.9 nm, zeta potential of -20.6 mV, encapsulation efficiency of 28.8 % and payload of 5.1 %). At a SIL concentration of 10 µg/mL, Lip-SIL exhibited the highest radioprotection for lymphocytes, but showed no radioprotection or even increased genotoxicity in human lung cancer A549 cells. Conclusion: Lip-SIL is a potential protector of human lymphocytes during radiotherapy in the treatment of non-small lung cancer. Moreover, the results of this study also imply that the radioprotection ability of bioactive compounds for normal cells is not only based on their scavenging activity on reactive oxygen species (ROS) but also on their mechanisms of intracellular activations. </div>     Liposomes, lung cancer, radioprotection, radiotherapy, silibinin. 521 529 http://ijrr.com/browse.php?a_code=A-10-1-940&slc_lang=en&sid=1 M.H. Nguyen jackminhhiep@yahoo.com 7900319475328460021930 7900319475328460021930 Yes Center of Radiation Technology and Biotechnology, Nuclear Research Institute, Dalat city, Lam-dong province, Vietnam N.D. Pham 7900319475328460021931 7900319475328460021931 No Center of Radiation Technology and Biotechnology, Nuclear Research Institute, Dalat city, Lam-dong province, Vietnam Q.T. Che 7900319475328460021932 7900319475328460021932 No Center of Radiation Technology and Biotechnology, Nuclear Research Institute, Dalat city, Lam-dong province, Vietnam T.H.N. Nguyen 7900319475328460021933 7900319475328460021933 No Department of Biology, Dalat University, Dalat city, Lam-dong province, Vietnam N.B.D. Vu 7900319475328460021934 7900319475328460021934 No Center of Radiation Technology and Biotechnology, Nuclear Research Institute, Dalat city, Lam-dong province, Vietnam T.N.M. Tran 7900319475328460021935 7900319475328460021935 No Center of Radiation Technology and Biotechnology, Nuclear Research Institute, Dalat city, Lam-dong province, Vietnam T.K. Trinh 7900319475328460021936 7900319475328460021936 No Department of Biology and Environment, Yersin University, Dalat city, Lam-dong province, Vietnam N.A. Trinh 7900319475328460021937 7900319475328460021937 No Department of Agriculture and Aquaculture, Tra Vinh University, Tra Vinh province, Vietnam B.N. Pham 7900319475328460021938 7900319475328460021938 No Center of Radiation Technology and Biotechnology, Nuclear Research Institute, Dalat city, Lam-dong province, Vietnam V.D. Le 7900319475328460021939 7900319475328460021939 No The Agriculture and Rural Development Department of Tra Vinh