International Journal of Radiation Research

en 68-Ga-labeled NODAGA-RGD-BBN heterodimer peptide as a novel radiotracer for dual integrin and GRPR-targeted tumor imaging Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Due to the excellent physical and biochemical characteristics of 68Ga, 68Ga-1,4,7-triazacy–clononane–glutaric acid– 4,7 acetic acid- arginyl-glycyl-aspartic acid- bombesin (68Ga-NODAGA-RGD-BBN) was prepared as a new positron emission tomography (PET) imaging agent, and afterward, the preclinical evaluation of this labeled peptide was studied. Materials and Methods: 68Ga radioisotope was extracted from a 68Ge/68Ga generator with high radionuclide, chemical and radiochemical purities. Then; the 68Ga-NODAGA-RGD-BBN radiolabeled complex was prepared at optimized conditions. The stability of the complex was evaluated in phosphate-buffered saline (PBS) for at least 2 h. Cell studies of the radiolabeled peptide were also assessed on the <a href="https://www.ncbi.nlm.nih.gov/gene/2925">gastrin releasing peptide receptor (</a>GRPR)-expressing cell line. Finally; the biodistribution and whole-body scan imaging study of 68Ga-NODAGA-RGD-BBN was studied in normal and tumor-bearing mice. Results: The biodistribution and whole-body scan imaging of the radiolabeled compound on GRPR-expressing tumor-bearing mice demonstrated the high uptake in the tumor site at all post-injection intervals.  The biodistribution results also demonstrated the major excretion route of the complex is the urinary tract. Conclusions: 68Ga-NODAGA-RGD-BBN shows high potential for PET imaging of patients with GRPR-expressing tumors; however, more biological studies are still needed.</div> GRPR positive tumors, radiolabeling, heterodimer peptide, PET imaging. 361 367 http://ijrr.com/browse.php?a_code=A-10-1-1053&slc_lang=en&sid=1 N. Amraee 7900319475328460024008 7900319475328460024008 No Medical Radiation Engineering Department, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran B. Alirezapour balirezapour1358@gmail.com 7900319475328460024009 7900319475328460024009 Yes Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran M. Hosntalab 7900319475328460024010 7900319475328460024010 No Medical Radiation Engineering Department, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran A. Hadadi 7900319475328460024011 7900319475328460024011 No Medical Radiation Engineering Department, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran H. Yousefnia 7900319475328460024012 7900319475328460024012 No Radiation Application Research School, Nuclear Science and Technology Research Institute (NSTRI), Tehran, Iran