en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The development of neurotoxicity in healthy, non-targeted brain tissue exposed to radiation during cranial radiotherapy (RT) is the most frequent event of radiation-induced adverse effects. The 5-hydroxytryptamine-3 (5-HT3) receptor antagonists may also have a range of neuroprotective, anti-inflammatory, and antiphlogistic properties in addition to their anti-emetic effects. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Study groups were formed in the following ways: Group 2: Irradiation (IR)-only (IR+Saline); Group 1: Normal control (orally fed control); Group 3: IR+Granisetron (IR+Granisetron): whole-brain IR and Granisetron 1 mg/kg/day (Merck) administered orally. 15 days of all therapies were given. The 15 days were completed with behavioral testing. In the entire brain IR-only (placebo) group, a substantial deterioration was seen in all studied marker levels and behavioral test results. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Compared to the IR-only group, all of these biochemical indicators significantly improved in the granisetron group (IR+Granisetron), and levels of the control group returned to normal. In behavioral test analyses, a substantial decline in the open field and passive avoidance learning social recognition tests was seen in the IR-only group compared to the healthy control group, whereas an improvement was seen in the IR+Granisetron group. In addition, the IR-only group showed a reduction in hippocampus neurons and Purkinje neurons as well as an increase in hippocampal gliosis, whereas the IR+Granisetron group showed an improvement and a return to the normal control group counts. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US"> In summary, we discovered that granisetron had neuroprotective properties in a rat model of radiation-induced brain damage.</span></span></span></span></span></span></span></span></span></div> Irradiation, granisetron, brain injury, 5-HT3, BDNF, SV2A, neuroinflammation. 435 446 http://ijrr.com/browse.php?a_code=A-10-1-1063&slc_lang=en&sid=1 N. Cini 7900319475328460025406 7900319475328460025406 No Department of Radiation Oncology Clinic, Kartal Dr. Lütfi Kırdar City Hospital, Istanbul, Turkey O. Atasoy 7900319475328460025407 7900319475328460025407 No Department of Radiation Oncology Clinic, Kartal Dr. Lütfi Kırdar City Hospital, Istanbul, Turkey M.A. Erdogan alpero86@gmail.com 7900319475328460025408 7900319475328460025408 Yes Department of Physiology, Izmir Katip Çelebi University, Faculty of Medicine, Izmir, Turkey G. Yaprak 7900319475328460025409 7900319475328460025409 No Department of Radiation Oncology Clinic, Kartal Dr. Lütfi Kırdar City Hospital, Istanbul, Turkey E. Eroglu 7900319475328460025410 7900319475328460025410 No Department of Histology and Embryology, Ege University, Faculty of Medicine, Izmir, Turkey C. Sirin 7900319475328460025411 7900319475328460025411 No Department of Histology and Embryology, Ege University, Faculty of Medicine, Izmir, Turkey Y. Uyanikgil 7900319475328460025412 7900319475328460025412 No Department of Histology and Embryology, Ege University, Faculty of Medicine, Izmir, Turkey O. Erbas 7900319475328460025413 7900319475328460025413 No Department of Physiology, Demiroğlu Bilim University, Faculty of Medicine, Istanbul, Turkey