International Journal of Radiation Research

en Neuroprotective effect of polyethylene Glycol (PEG-3350) on radiation-induced brain injury via membrane stabilization Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: This research aims to evaluate whether polyethylene glycol (PEG), which can immediately repair neuronal membranes, will have a protective effect against radiation-induced brain injury (RIBI). Materials and Methods: Whole brain irradiation (RAD) procedure was performed on 14 of 21 rats included in the study for establishing RIBI. Rats were randomly divided into three groups containing equal numbers of animals: control (RAD not established), placebo (RAD applied, and treated with 1 ml/kg/day of saline that was administered intraperitoneally), and treatment (RAD applied, and treated with 30 mg/kg /day of PEG 3350 that was administered intraperitoneally). Following the end of the 22-day treatments, behavioral tests were performed first, and then magnetic resonance spectroscopy (MRS) was applied to measure brain lactate levels. All rats were sacrificed in order to perform biochemical analysis and histopathological examination of brain tissue. Results: Compared to the control group, MDA, TNF-α, NF-κB and lactate levels were significantly increased, while BDNF levels were decreased in the placebo group; and RAD-induced changes in all these biochemical markers were reversed by PEG. In MRS performed from the corpus striatum, there was an evident decrease in lactate levels after PEG treatment. Impairment in memory and learning was more limited in PEG-treated rats than in the rats receiving saline therapy. Furthermore, histological examinations from hippocampus and cerebellum exhibited that PEG treatment significantly reduces apoptosis, astrogliosis, and oxidative DNA damage in rats. Conclusion: Our results support that PEG is very effective in preventing RIBI with its secondary effects on the basis of membrane stabilization.</div> Radiation, PEG-3350, MRS, 8-oxo-dG, BDNF, membrane stabilization. 733 743 http://ijrr.com/browse.php?a_code=A-10-1-1114&slc_lang=en&sid=1 I.H. Sever 7900319475328460025414 7900319475328460025414 No Demiroglu Bilim University, School of Medicine, Department of Radiology, Istanbul, Turkey B. Ozkul 7900319475328460025415 7900319475328460025415 No Istanbul Atlas University, School of Medicine, Department of Radiology, Istanbul, Turkey O. Atasoy 7900319475328460025416 7900319475328460025416 No Kartal Dr. Lutfi Kirdar Training and Research Hospital, Department of Radiation Oncology, Istanbul, Turkey N. Cini 7900319475328460025417 7900319475328460025417 No Kartal Dr. Lutfi Kirdar Training and Research Hospital, Department of Radiation Oncology, Istanbul, Turkey M.F. Bozkurt 7900319475328460025418 7900319475328460025418 No Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pathology, Afyonkarahisar, Turkey 5Izmir Katip Celebi University, Department of Physiology, Izmir, Turkey M.A. Erdogan alpero86@gmail.com 7900319475328460025419 7900319475328460025419 Yes Izmir Katip Celebi University, Department of Physiology, Izmir, Turkey G. Yaprak 7900319475328460025420 7900319475328460025420 No Kartal Dr. Lutfi Kirdar Training and Research Hospital, Department of Radiation Oncology, Istanbul, Turkey O. Erbas 7900319475328460025421 7900319475328460025421 No Demiroğlu Bilim University, School of Medicine, Department of Physiology, Istanbul, Turkey