en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Wireless Fidelity (Wi-Fi) exposure might induce tissue damage through non-thermal effects. Nonetheless, only a few studies have evaluated the non-thermal effects on the testis undergoing reproductive development following Wi-Fi exposure. This study aimed to assess the oxidative stress status in the testis and subsequent sperm quality of growing rat pups that received edible bird nest (EBN) supplementation simultaneously. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Fourteen weeks of 2. 45GHz Wi-Fi exposure and simultaneous 250mg/kg EBN were supplemented to three-week-old male Sprague&ndash;Dawley rats. Thirty animals were divided equally into control, control EBN, Wi-Fi, sham Wi-Fi, and Wi-Fi+EBN. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Results showed an increase in total oxidant status (TOS), a significant decrease in total antioxidant status (TAS), and a significant increase in the expression of 8-hydroxy-2&prime;-deoxyguanosine (8-OHdG) in the testis of the Wi-Fi group. Sperm chromatin integrity, morphology, concentration, and motility were among the parameters that significantly decreased. Supplementation of 250mg/kg EBN in the Wi-Fi+EBN group significantly reduced TOS and 8-OHdG expression in the testis. EBN supplementation also significantly increased sperm chromatin integrity, morphology, and concentration. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Wi-Fi exposure induces oxidative stress in the testis and affects important sperm parameters in rat pups. EBN supplementation quenched the oxidative stress activity due to Wi-Fi exposure and improved sperm quality.</span></span></span></span></span></span></span></span></span></div> IMRT, OARs, TPS, DVH, mixed energy plans. 329 338 http://ijrr.com/browse.php?a_code=A-10-1-1178&slc_lang=en&sid=1 F.H.F. Jaffar 7900319475328460026913 7900319475328460026913 No Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia K. Osman 7900319475328460026914 7900319475328460026914 No Forensic Science Program, Centre for Diagnostic Science & Applied Health, Faculty of Health Sciences, Universiti Kebangsaan Malaysia (UKM), UKM Bangi, Selangor 43600, Malaysia C.K. Hui 7900319475328460026915 7900319475328460026915 No Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia A.F. Zulkefli 7900319475328460026916 7900319475328460026916 No Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia S.F. Ibrahim timi@ukm.edu.my 7900319475328460026917 7900319475328460026917 Yes Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia (UKM), Jalan Yaacob Latif, Bandar Tun Razak, Cheras, Kuala Lumpur 56000, Malaysia