International Journal of Radiation Research

en MiR-144-3p regulates invasion and proliferation activity in hepatocellular carcinoma by targeting SLITRK4 Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: miR-144-3p exerts inhibitory roles in hepatocellular carcinoma (HCC). This paper aims to explore that miR-144-3p and its predictive target gene, SLIT- and NTRK-like family member 4 (SLITRK4), in HCC progression. Materials and Methods: The levels of SLITRK4 and miR-144-3p were determined by western blot and qPCR. A loss- and gain-of-function test was used to test whether the relationship between miR-144-3p and SlitrK4 affected the growth of HCC cells. StarBase examined the miR-144-3p target prediction using SLITRK4. Through the use of a luciferase reporter experiment, miR-144-3p's targeting SLITRK4 was identified. The salvage experiment confirmed that miR-144-3p interacts SLITRK4 to regulate the progression of HCC. Results: Findings showned that the expression of miR-144-3p decreased in HCC tissue and cells. MiR-144-3p inhibits deterioration progression of HCC cell progression. MiR-144-3p targeted SLITRK4 and negatively modulated SLITRK4 levels. Besides, miR-144-3p upregulation decreased HCC migration and invasion activity, while SLITRK4 overexpression reduced this inhibition effects. Conclusions: MiR-144-3p regulates invasion and proliferation activity in HCC by targeting SLITRK4. MiR-144-3P/ SLITRK4 axis might provide a potential anti-cancer therapy pathway for clinical diagnosis, treatment and prognosis in hepatoma.</div> MiR-144-3p, SLITRK4, tumor progression, Hepatocellular carcinoma. 361 366 http://ijrr.com/browse.php?a_code=A-10-1-1181&slc_lang=en&sid=1 D.B. Xiao 7900319475328460026956 7900319475328460026956 No Department of Clinical Laboratory, SiJing Hospital of SongJiang District, Shanghai City, 201601, China H. Xu 7900319475328460026957 7900319475328460026957 No Department of Oncology, Ezhou Central Hospital, Ezhou City, Hubei Province, 436000, China J.C. Li 7900319475328460026958 7900319475328460026958 No Department of Clinical Laboratory, Hengyang Central Hospital, Hengyang City, Hunan Province, 421000, China K.K. Peng 7900319475328460026959 7900319475328460026959 No Department of Clinical Laboratory, SiJing Hospital of SongJiang District, Shanghai City, 201601, China X.L. Jiang 7900319475328460026960 7900319475328460026960 No Department of Clinical Laboratory, SiJing Hospital of SongJiang District, Shanghai City, 201601, China D.X. Wang 7900319475328460026961 7900319475328460026961 No Department of General Surgery, SiJing Hospital of SongJiang District, Shanghai City, 201601, China F.F. Zhu zff200078@163.com 7900319475328460026962 7900319475328460026962 Yes Department of Hepatobiliary and Pancreas Surgery, The First Affiliated Hospital of University of South China, Hengyang City, Hunan Province, 421001, China