en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">MicroRNAs (miRNAs) have crucial roles in human cancers. Many studies have certified that miRNAs are implicated in tumor progression via exosomes. Nevertheless, whether miRNA-339 is derived by exosomes and its effects in gastric cancer (GC) presents obscure. Therefore, our study attempted to clarify the functional role and molecular mechanism of miRNA-339 in GC. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">In this research, the potential of miRNA-339 in GC was verified through miRNA-339 elevation with cell function assays. Bioinformatics analysis together with mechanical assay was implemented for assessing the regulatory relation between miRNA-339 and zinc finger protein 689 (ZNF689). Moreover, the existence of exosomes was determined via transmission electron microscopy together with nanoparticle tracking analysis. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">miRNA-339 presented significant down-regulation in GC. miRNA-339 elevation suppressed GC cell proliferation, invasion along with migration while elevated GC cell apoptosis. miRNA-339 targeted ZNF689 3&rsquo;UTR to repress ZNF689 expression, thereby hindering GC progression. Finally, miRNA-339 was majorly incorporated into exosomes to hinder GC progression. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">In summary, exosome-delivered miRNA-339 may act to be a tumor repressor in GC by targeting ZNF689, which might be an underlying therapeutic target for GC.</span></span></span></span></span></span></span></span></span></div> Gastric cancer, miRNA-339, ZNF689, exosome. 443 453 http://ijrr.com/browse.php?a_code=A-10-1-1193&slc_lang=en&sid=1 L. Liang 7900319475328460028960 7900319475328460028960 No Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China Z. Zhang 7900319475328460028961 7900319475328460028961 No Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China Z. Cheng 7900319475328460028962 7900319475328460028962 No Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China H. Li 7900319475328460028963 7900319475328460028963 No Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China H. Jiang jianghouxiang2023@163.com 7900319475328460028964 7900319475328460028964 Yes Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China