International Journal of Radiation Research

en The role of m6A-related lncRNAs on prognosis and chemo radiotherapy response of osteosarcoma: potential molecular pathways Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Conventional treatments on osteosarcoma (OS) yield limited improvements in metastatic or relapsed cases, necessitating innovative therapeutic strategies. This study's objective is to reveal the role of long non-coding RNAs (lncRNAs) in prognosis and potential molecular pathways of OS. Materials and Methods: This study integrated bioinformatics, statistical analysis, and computational techniques to investigate m6A-related prognostic lncRNA clusters in OS. Consensus clustering and risk analysis were performed on the m6A-related prognostic lncRNAs. Nomogram model was built for survival prediction, and was assessed using survival time receiver operator characteristic (ROC) analysis and decision curves. A competitive endogenous RNA (ceRNA) network was built to explore lncRNA-miRNA (microRNA)-mRNA interactions using the microRNA database and metascape website, followed by functional enrichment analyses. Results: The analysis unveiled three distinct OS clusters, with Cluster 2 exhibiting the highest risk in the immune microenvironment. High risk 7-lncRNA signature demonstrated strong prognostic value in predicting OS overall survival. The ceRNA network highlighted complex interactions among mRNAs, miRNAs and lncRNAs. Functional enrichment analysis indicated that mRNAs within the ceRNA network were linked to many biological functions. Furthermore, 30 differentially expressed genes (DEGs) derived from low-risk and high-risk groups enriched in GO-BP connected to MAGE (melanoma antigen gene) family member A2 (MAGEA2) and MAGEA2B. These DEGs in high risk group were also upregulated in the three KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway. Conclusion: This study disclosed the role of high-risk m6A-associated lncRNAs in prognosis and potential molecular pathways of OS. These findings provided novel insights into OS pathogenesis and potential therapeutic targets, warranting further validation in clinical settings.</div> osteosarcoma, long non-coding RNA (lncRNA), m6A modification, prognosis, chemoradiotherapy response, ceRNA network. 463 471 http://ijrr.com/browse.php?a_code=A-10-1-1195&slc_lang=en&sid=1 Y. Zhou 7900319475328460028681 7900319475328460028681 No Department of Anesthesiology, Dongfang Hospital of Beijing University of Chinese Medicine, Beijing, 100078, China Y. Bi 7900319475328460028682 7900319475328460028682 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China M. Wan 7900319475328460028683 7900319475328460028683 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China N. Xu 7900319475328460028684 7900319475328460028684 No Department of Orthopaedics, Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200233, China Y. Xu 7900319475328460028685 7900319475328460028685 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China P. Liu 7900319475328460028686 7900319475328460028686 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China H. Jiang 7900319475328460028687 7900319475328460028687 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China H. Fang 7900319475328460028688 7900319475328460028688 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China H. Hu 7900319475328460028689 7900319475328460028689 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China S. Xu xusuyang 7900319475328460028690 7900319475328460028690 Yes Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China S. Lan lanshenghui_gk@163.com 7900319475328460028691 7900319475328460028691 No Department of Orthopaedics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China