en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">To systematically evaluate the effects of radiofrequency ablation on lymphocytes, subsets, and cytokines in patients with thyroid cancer. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Method:</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US"> Radiofrequency ablation, thyroid cancer, lymphocytes, and other key terms are used to search for randomized controlled clinical trials (RCTs) related to them.The data analysis is conducted using RevMan 5.3 software. The relative risk (RR) is used to analyze the effect magnitude of binary variable data. The mean difference (MD) represents the continuous variable data results. The interval estimation is presented as 95% CI. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The standardized mean difference between the CD3+, CD4+, sub-population counts, and CD4+/CD8+ratios among the studies was 1.91 (95% CI0.91 ~ 2.91), 0.07(95% CI-0.07 ~ 0.22). The combined effect quantity indicated that radiofrequency ablation had a significant effect on improving lymphocytes and sub-populations in thyroid cancer patients.The heterogeneity test results among different studies were I2 = 99%, 99%, P < 0.01 and 0.32. The results indicated a high level of heterogeneity between the two groups. The standardized mean difference of cytokine levels such as IL-6 and TNF-a in different studies was -4.20 (95% CI-4.30 ~ -4.10), -6.40 (95% CI-7.07 ~ -5.77). The combined effect quantity indicated that radiofrequency ablation had a significant effect on reducing serum inflammatory cytokine levels in thyroid cancer patients.The heterogeneity test results between different studies were I2 = 100%, 97%, and P < 0.01. The results indicated a high level of heterogeneity between the two groups. The differences between groups are statistically significant (x2 = 4.48, P < 0.01). </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Compared with other surgeries, radiofrequency ablation can inhibit CD4+, promote CD8+lymphocyte proliferation, and reduce inflammatory factors in thyroid cancer patients.</span></span></span></span></span></span></span></span></span></div> Thyroid cancer, Radiofrequency ablation, Lymphocytes and sub-populations, Cytokines, Meta-analysis. 1 12 http://ijrr.com/browse.php?a_code=A-10-1-1285&slc_lang=en&sid=1 B. Miao 7900319475328460033076 7900319475328460033076 No Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China G. Ruan ruangangqiang0711@126.com 7900319475328460033077 7900319475328460033077 Yes Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China F. Meng 7900319475328460033078 7900319475328460033078 No Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China Q. Zhang 7900319475328460033079 7900319475328460033079 No Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China J. Pei 7900319475328460033080 7900319475328460033080 No Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China D. Li 7900319475328460033081 7900319475328460033081 No Breast & Thyroid & Head and Neck Surgery, Affiliated Hospital of Shaoxing University, Shaoxing, 312000, China