en Nuclear Medicine Nuclear Medicine تحقيق بديع Original Research <span style="font-weight: bold">Background:</span> Proton induced X-ray emission (PIXE) has been applied as reliable and improved techniques in this study to compare concentration of various trace elements in normal and abnormal varicose veins. <span style="font-weight: bold">Materials and Methods:</span> Five samples from normal veins and 13 samples from varicose veins bombarded by 2.0 MeV energy proton beams produced by a Van de Graff accelerator in vacuum. Two specimens from each sample, approximately 1cm, were processed for PIXE (proton induced X-ray emission) analysis. Both qualitative and quantitative analyses of potassium, iron, copper and zinc concentration were performed with respect to calcium concentration. <span style="font-weight: bold">Results: </span>The concentration of potassium and iron in the varicose vein group has been significantly higher than the normal group. Copper and zinc concentration were also higher in the varicose veins, and the elevation of bromine was seen in the normal group. <span style="font-weight: bold">Conclusion:</span> PIXE analysis showed higher concentrations of trace elements in veins derived from varicos patients compared to normal group. The difference in normal and abnormal vein might be independent of age. Iran. J. Radiat. Res., 2010 8 (2): 117-121 Varicose vein, trace elements, PIXE. 117 121 http://ijrr.com/browse.php?a_code=A-10-1-359&slc_lang=en&sid=1 K. Moosavi kmoosavi@aeoi.org.ir 790031947532846001826 790031947532846001826 Yes S. Vatankhah 790031947532846001969 790031947532846001969 No J. Salimi 790031947532846001970 790031947532846001970 No M. Moradi 790031947532846001971 790031947532846001971 No