en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The current study aimed to investigate the ability of acetylsalicylic acid (ASA) to prevent ionizing radiation-induced DNA damage in human lymphocytes via the micronucleus (MN) assay and compare it with the efficacy of the agent WR-1065 (2-[(3-Aminopropyl) amino] ethanethiol dihydrochloride), which exhibits radioprotective activity. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods:</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US"> Peripheral blood samples were obtained from four participants, and nine experimental groups were established. Blood samples were treated with 25 &micro;g/mL ASA and 40 &micro;g/mL WR-1065 for 30 min before irradiation. After treatment, the samples were irradiated with 6 MV X-rays in the linear accelerator (LINAC) unit at doses of 2 Gy and 4 Gy. All blood samples were then cultured for the micronucleus assay. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">In vitro treatments with ASA and WR-1065 at the indicated doses did not result in statistically significant changes in MN frequencies (p˃0.05). </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Both drugs were did not exhibit radioprotective effects in the experimental model used.</span></span></span></span></span></span></span></span></span></div> Acetylsalicylic acid, WR-1065, micronucleus, radioprotection, ionizing radiation 435 439 http://ijrr.com/browse.php?a_code=A-10-1-1347&slc_lang=en&sid=1 A. Yalcin aydoganyalcin@gmail.com 7900319475328460030788 7900319475328460030788 Yes Department of Radiation Oncology, Faculty of Medicine, Trakya University, Edirne, Turkey K. Tabakcioglu 7900319475328460030789 7900319475328460030789 No Department of Medical Biology, Faculty of Medicine, Trakya University, Edirne, Turkey V.Y. Caloglu 7900319475328460030790 7900319475328460030790 No Department of Radiation Oncology, Faculty of Medicine, Trakya University, Edirne, Turkey C. Uzal 7900319475328460030791 7900319475328460030791 No Department of Radiation Oncology, Faculty of Medicine, Near East University, Faculty of Medicine, Lefkoşa, Turkish Republic of Northern Cyprus I.K. Omurlu 7900319475328460030792 7900319475328460030792 No Department of Biostatistics, Faculty of Medicine, Adnan Menderes University, Aydın, Turkey