en Radiation Biology Radiation Biology تحقيق بديع Original Research <p><strong>Background: </strong>Copper-64 (T_1/2=12.7 h) is an important radionuclide used both in PET imaging and therapy. [⁶⁴Cu]-pyruvaldehyde-<i>bis </i>(N⁴-methylthiosemicarbazone) ([⁶⁴Cu]-PTSM) is one of the most famous copper radiopharmaceuticals with unique specifications (suitable half life, <br>stability, etc.). The wide range of ⁶⁴Cu applications arouse great interest for its production. </p><p><strong>Materials and Methods: </strong>Cu-64 was produced <i>via</i> the ⁶⁸Zn (p, α n) ⁶⁴Cu nuclear reaction and isolated from the irradiated target by a two-step chemical method. [⁶⁴Cu]-PTSM was prepared using in-house made PTSM ligand and [⁶⁴Cu] cuprous acetate. The complex formation parameter s (time, temperature, concentration and elution methods) were determined carefully. </p><p><strong>Results: </strong>Copper-64 was prepared in chloride form (≈200 mCi, >95% chemical yield at 180 μA for 1.1 h irradiation, radionuclidic purity >96%, copper-67 as impurity). The solution of ⁶⁴Cu-PTSM was prepared in >80% radiochemical yield and more than 98% radiochemical purity. Quality controls and stability tests were performed for the final solution. </p><p><strong>Conclusion: </strong>[⁶⁴Cu]-PTSM was prepared at the radiopharmaceutical scales with high quality and potential to be used in therapeutic/imaging centers. <i>Iran </i><i>. J. Radiat. Res., 2004 2 (3): 107-115 </i></p> ,hypoxia imaging, cyclotron,targeted therapy, PTSM,Copper-64,PET 107 115 http://ijrr.com/browse.php?a_code=A-10-1-66&slc_lang=en&sid=1 A.R. Jalilian ajalilian@nrcam.org 79003194753284600162 79003194753284600162 Yes P. Rowshanfarzad 79003194753284600163 79003194753284600163 No J. Moafian 79003194753284600164 79003194753284600164 No M. Kamali-dehghan 79003194753284600165 79003194753284600165 No M. Mirzaii 79003194753284600166 79003194753284600166 No