en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">Iodine-125 (</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I) brachytherapy is an effective strategy for treating human tumors. The current study aimed to discover the mechanisms underlying the therapeutic action of</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I radiation in lung cancer, with a focus on its impact on the epithelial-mesenchymal transition (EMT). </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">A549 cells, a human lung adenocarcinoma cell line, were treated with transforming growth factor </span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1 (</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1) </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">and/or </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I (control, TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1, </span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I, and TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1 + </span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I groups) to evaluate the effects of </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I on TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1-</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">induced EMT. After treatment, the viability of A549 cells was detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The expression of E-cadherin, N-cadherin, Vimentin, Smad3, and Snai1 markers and pathway molecules was measured. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I radiation inhibited the viability of A549 cells, both with and without TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1 </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">treatment. TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1 </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">intervention promoted the EMT of A549 cells, as demonstrated by the morphological transition from a polygonal shape to a spindle shape, reduced E-cadherin levels, and elevated Vimentin and N-cadherin expression. Notably, TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1-</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">activated EMT was significantly weakened by </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I radiation. Moreover, </span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">¹²⁵</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">I radiation reversed TGF-</span></span></span><span lang="el" style="font-family:Calibri"><span style="color:black"><span style="language:el">&beta;1-</span></span></span><span lang="en-US" style="font-family:Calibri"><span style="color:black"><span style="language:en-US">induced upregulation of Smad3 and Snai1 in A549 cells. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-family:Calibri"><span style="language:en-US">¹²⁵</span></span><span lang="en-US" style="language:en-US">I radiation</span> <span lang="en-US" style="font-family:Calibri"><span style="color:#222222"><span style="language:en-US">suppresses the</span></span></span><span lang="en-US" style="language:en-US"> EMT by blocking TGF-</span><span lang="el" style="font-family:Calibri"><span style="language:el">&beta;1/</span></span><span lang="en-US" style="language:en-US">Smad3/Snai1 signaling, contributing to the treatment of lung cancer.</span></span></span></span></span></span></span></div> Lung neoplasms, iodine-125, epithelial-mesenchymal transition, transforming growth factor beta1, smad3 protein. 751 756 http://ijrr.com/browse.php?a_code=A-10-1-1394&slc_lang=en&sid=1 F. Song 7900319475328460031915 7900319475328460031915 No Department of Minimally Invasive Intervention, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming, 650118, China J. Che 7900319475328460031916 7900319475328460031916 No Department of Minimally Invasive Intervention, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming, 650118, China H. Li 7900319475328460031917 7900319475328460031917 No Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming, 650118, China G. Zhu 7900319475328460031918 7900319475328460031918 No Department of Minimally Invasive Intervention, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming, 650118, China M. Huang 7900319475328460031919 7900319475328460031919 No Department of Hepatobiliary Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Peking University Cancer Hospital Yunnan, Kunming, 650118, China D. Xu 632533054@qq.com 7900319475328460031920 7900319475328460031920 Yes Department of Medical Laboratory, Pingbian County People's Hospital, Pingbian 661200, Yunnan, China