en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Chemotherapy resistance has become a problem for the treatment of breast cancer patients. This study aimed to investigate whether astragaloside IV (AS-IV) has synergistic effects to enhance Paclitaxel (PTX) chemosensitivity in breast cancer cells. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">CCK-8 assays were performed to evaluate the sensitivity of the cell to AS-IV and PTX. The flow cytometric analysis of Annexin-V-fluoresceine isothiocyanate (FITC)/propidium iodide (PI) staining was carried out to evaluate cell apoptosis. The western blotting assay was used to measure the apoptosis-related proteins and Nrf-2 levels. ROS levels were measured using Dichlorofluorescein (DCF) assay. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">AS-IV enhances PTX chemosensitivity in MDA-MB-231 and MCF-7 cells by enhancing the inhibitory effect of cell viability and promotion effect on cell apoptosis of PTX. The synergistic effects of AS-IV to PTX could decrease the Nrf-2 expression and increase the ROS levels. Nrf-2 signaling was related to ROS generation. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">AS-IV may enhance PTX chemosensitivity in breast cancer via regulating Nrf-2 signaling. AS-IV might be used as a potential adjuvant drug to increase PTX sensitivity in breast cancer cells.</span></span></span></span></span></span></span></span></span></div> Astragaloside IV, breast cancer, chemosensitivity, nuclear factor erythroid 2-related factor 2, reactive oxygen species. 1065 1070 http://ijrr.com/browse.php?a_code=A-10-1-1437&slc_lang=en&sid=1 T. Runwei 7900319475328460032538 7900319475328460032538 No General Surgery Department, Liqun Hospital, Putuo District, Shanghai, China L. Chaoran 7900319475328460032539 7900319475328460032539 No Breast Surgery/Medical Department, Baoshan District Integrated Traditional Chinese and Western Medicine Hospital, Shanghai, China Z. Yinghui postop.tsin@foxmail.com 7900319475328460032540 7900319475328460032540 Yes Breast Surgery Department, Jiading Maternal and Child Health Hospital, Shanghai, China