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AWT IMAGE

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Volume 12, Issue 4 (10-2014)                   Int J Radiat Res 2014, 12(4): 377-382 | Back to browse issues page

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Shourian M, Ghourchian H. Comparison between Amperometric and Chemiluminescence methods in detection and dosimetry of cobalt-60 gamma ray emission . Int J Radiat Res 2014; 12 (4) :377-382
URL: http://ijrr.com/article-1-1359-en.html
Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran , hadi@ibb.ut.ac.ir
Abstract:   (7444 Views)

Background: Hydrogen peroxide (H2O2), produced by gamma ray irradiation to watery solution, was used as an analytical parameter for dosimetry of cobalt-60 (60Co) radiation. Materials and Methods: Detection of the produced H2O2 was carried out using two methods: an amperometric biosensor fabricated by immobilization of anthraquinone 2-carboxylic acid modified horseradish peroxidase on glassy carbon electrode, and chemiluminescence technique using luminol as a fluorophore and diperiodatocuprate as a catalyst. Results: In the first method, at the applied potential of -550 mV (vs. Ag/AgCl) the biosensor showed the sensitivity of 74.46 nA/Gray and detection limit of 0.061 Gray (R.S.D=3.67% for N=3) towards H2O2 produced by 60Co. In the second method, the sensitivity and detection limit for 60Co gamma ray was determined to be 2.34 (luminescence intensity/Gray) and 0.069 Gray (R.S.D=4.16% for N=3), respectively. Conclusion: The obtained results revealed that both proposed techniques, with leaner range from 0.25 to 5 Grays are applicable for detection and dosimetry of gamma ray from 60Co.

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Type of Study: Short Report | Subject: Radiation Biology

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