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:: Volume 14, Number 3 (7-2016) ::
IJRR 2016, 14(3): 181-188 Back to browse issues page
Radiation-induced expression of platelet endothelial cell adhesion molecule-1 in cerebral endothelial cells
B. Chen, Dr. Z. Zhao , V. Lee, R. Reddy, M. Stoodley
Macquarie University , zhenjun.zhao@mq.edu.au
Abstract:   (1083 Views)

Background: Radiation-induced molecular changes on the endothelial surface of brain arteriovenous malformations (AVM) may be used as markers for specific vascular targeting agents. In this study, we examined the level of expression of platelet endothelial cell adhesion molecule-1 (PECAM-1) on brain endothelial cell surface after radiation treatment, with the aim of targeting the radiation-induced PECAM-1 on the AVM endothelium with pro-thrombotic agents to selectively occlude AVM vessels. Materials and Methods: Mouse cerebral endothelial cells (bEnd.3) were irradiated with 5, 15, or 25 Gy. Real-time quantitative polymerase chain reaction (PCR) and in-cell enzyme-linked immunosorbent assay (ELISA) were performed to quantify the temporal gene and surface PECAM-1 protein expression up to 168 hours post-irradiation. Two-tailed unpaired t-tests were used to determine statistical significance. Results: PECAM-1 gene expression was found to be significantly elevated post-irradiation in real-time quantitative PCR, with the maximum level of gene expression being evident at 120 hours post-irradiation representing an 11-fold increase in comparison to non-irradiated controls (p<0.001). In-cell ELISA detected a similar up-regulation for protein expression on the cell surface with delayed peak time. Conclusion: Ionising radiation can induce the up-regulation of PECAM-1 on brain endothelial cell surface. This protein may be a potential candidate for facilitating selective AVM vessel occlusion through the application of radiosurgery followed by vascular targeting.

Keywords: Arteriovenous malformations (AVM), platelet endothelial cell adhesion molecule-1 (PECAM-1), radiation, cerebral endothelial cells.
Full-Text [PDF 630 kb]   (364 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
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DOI: 10.18869/acadpub.ijrr.14.3.181


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Chen B, Zhao Z, Lee V, Reddy R, Stoodley M. Radiation-induced expression of platelet endothelial cell adhesion molecule-1 in cerebral endothelial cells. IJRR. 2016; 14 (3) :181-188
URL: http://ijrr.com/article-1-1759-en.html
Volume 14, Number 3 (7-2016) Back to browse issues page
International Journal of Radiation Research
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