[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
For Authors::
For Reviewers::
Subscription::
News & Events::
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE

:: Volume 17, Issue 4 (10-2019) ::
Int J Radiat Res 2019, 17(4): 579-586 Back to browse issues page
Smad 2/3 and CD8+ distribution pattern in liver of mice vaccinated with gamma radiation-attenuated Schistosoma mansoni larvae
E.N. Hafez , M.M. Amin
Department of Health Radiation Research, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt , eman_naser612@hotmail.Com
Abstract:   (2541 Views)
Background: Gamma radiation-attenuated vaccine has a very promising value in controlling schistosomiasis. The objective of this study is to investigate the effects of vaccination with gamma radiation-attenuated schistosomules regarding histopathological and immunohistochemical changes in mice model of human Schistosoma mansoni infection. Materials and Methods: The study is conducted upon 40 mice, divided into four groups of 10 each: uninfected control group A (normal control), Schistosoma mansoni infected group B (infected control), Group C subcutaneously injected with 200 Gy gamma radiation–attenuated schistosomules (vaccine control) and group D injected with the same dose of gamma radiation-attenuated schistosomules then challenged after 4 weeks by S. mansoni cercariae (vaccinated-challenged). Parts of liver tissues stained with Masson's trichrome (MT) stain for classifying the granulomas into cellular, fibrocellular or fibrous according to the amount of collagens; stained bluish green. Immunohistochemistry assay was then done on the other portion through expression of Smad2/3 protein and CD8+ in the extracellular matrix (ECM). Results: Compared to control infected group, mild histopathological changes with decrease of egg granulomas mainly cellular is detected in vaccinated-challenged group. This is accompanied with moderate immunohistochemical expression of Smad2/3 and CD8+ proteins in vaccine-control group and mild expression in vaccinated-challenged group. Conclusion: The The results indicate that vaccination with 200 Gy gamma-irradiated schistosomules could reduce histopathological and immunohistochemical changes induced due to infection which represents an effective strategy in disease control.
Keywords: Schistosoma mansoni, vaccination, immunohistochemistry, histochemistry.
Full-Text [PDF 3263 kb]   (847 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hafez E, Amin M. Smad 2/3 and CD8+ distribution pattern in liver of mice vaccinated with gamma radiation-attenuated Schistosoma mansoni larvae . Int J Radiat Res 2019; 17 (4) :579-586
URL: http://ijrr.com/article-1-2662-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 17, Issue 4 (10-2019) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.05 seconds with 50 queries by YEKTAWEB 4642