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:: Volume 17, Issue 4 (10-2019) ::
Int J Radiat Res 2019, 17(4): 617-624 Back to browse issues page
Topically applied melatonin ameliorates radiation-induced skin fibrosis in mice
D.H. Kim, Y.M. Choi, Y.K. Ki, K.S. Cho, Y.J. Choi, W.T. Kim Dr.
Department of Radiation Oncology, Otorhinolaryngology, and Hemato-oncology, Biomedical Research Institute, Pusan National University Hospital and Pusan National University School of Medicine, Pusan National University, Busan, Korea , rokwt@hanmail.net
Abstract:   (200 Views)
Background: We investigated whether topical administration of melatonin ameliorates radiation-induced skin fibrosis (RISF) and inhibits the expression of profibrogenic genes in mice. Materials and Methods: Forty-eight female BALB/c mice were randomly divided into three groups: topically applied 5% ethanol (Control), topically applied 5% ethanol plus irradiation (IR), and topically applied melatonin plus irradiation (Mel+IR). The right hind legs of the IR and Mel+IR group mice were exposed to two fractions of electron beam radiation (20 Gy × 2). For 4 weeks, melatonin solution (10 mg/day) was topically applied to Mel+IR group mice. Fourteen days after IR, the relative levels of transforming growth factor (TGF)-β1 mRNA expression and TGF-β1 protein in skin specimens were analyzed by real-time quantitative PCR and immunohistochemical staining. Dermal thickness and tissue collagen accumulation were measured at 100 days post irradiation. Results: The Radiation caused a 2.2-fold increase in TGF-β1 mRNA expression relative to that in control group, which was decreased by 37% following melatonin treatment (P = 0.024). We also observed substantial reduction of TGF-β1 expression in immunohistochemical studies. The mean values of dermal thickness were 105 ± 11 μm (Control), 195 ± 21 μm (IR), and 148 ± 19 μm (Mel+IR). Dermal thickness and collagen accumulation, which increased in the IR group, was significantly reduced by topically applied melatonin.  Conclusion: Topical administration of melatonin successfully attenuated RISF.
Keywords: Melatonin, Radiation, Fibrosis, Transforming growth factor-β, Topical application.
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Type of Study: Original Research | Subject: Radiation Biology
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Kim D, Choi Y, Ki Y, Cho K, Choi Y, Kim W. Topically applied melatonin ameliorates radiation-induced skin fibrosis in mice . Int J Radiat Res. 2019; 17 (4) :617-624
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Volume 17, Issue 4 (10-2019) Back to browse issues page
International Journal of Radiation Research
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