[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 18, Issue 1 (1-2020) ::
Int J Radiat Res 2020, 18(1): 65-74 Back to browse issues page
Caveolin-1 was involved in the reduced adiosensitivity to X-ray in human mammary epithelial MCF10A cells
Y.J. Zhang, H.Y. Li, C. Qu, N. Zhang, J. Liu, Y.Y. Cui, W. Zou PhD.,
Abstract:   (304 Views)
Background: X-ray chest fluoroscopy is a compulsory component of the health examination procedure in China. The radiation dose from chest fluoroscopy is the largest in X-ray examination. More than half of the women in their twenties with breast cancer have been given X-ray fluoroscopy. Studies have shown that Caveolin-1 is involved in the repair of damage DNA in tumor cells induced by irradiation. However the mechanism and role of Caveolin-1 in normal human mammary epithelial cells are not clear.  Materials and Methods: Here, normal human mammary epithelial cells (MCF10A) and the cells with Caveolin-1 knockdown (MCF10ACE) were exposed to X-ray radiation to investigate the role of Caveolin-1 in the enhancement of radiosensitivity in these cells and the associated mechanism. Results: Decreased survival rate and a significantly higher level of cell arrest at the G1 and G2 phases, as well as reduced activation of the DNA damage repair proteins ATM and p53, and the stress protein p38MAPK were manifested by MCF10ACE cells compared to MCF10A cells, following exposure to X-ray radiation. Furthermore, binding between Caveolin-1 and Mdm2 in MCF10ACE cells was also lower than in MCF10A cells. Conclusion: Overall, the finding indicated that Caveolin-1 played an important role in decreasing the radiosensitivity of human mammary epithelial cells.
Keywords: Caveolin-1, mammary epithelial cell, radiosensitivity enhancement, DNA damage repair.
Full-Text [PDF 2155 kb]   (50 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zhang Y, Li H, Qu C, Zhang N, Liu J, Cui Y et al . Caveolin-1 was involved in the reduced adiosensitivity to X-ray in human mammary epithelial MCF10A cells. Int J Radiat Res. 2020; 18 (1) :65-74
URL: http://ijrr.com/article-1-2777-en.html

Volume 18, Issue 1 (1-2020) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.06 seconds with 34 queries by YEKTAWEB 4056