[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 2, Issue 1 (6-2004) ::
Int J Radiat Res 2004, 2(1): 15-20 Back to browse issues page
Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry
S. Hosseini, H. Mozdarani
Abstract:   (20494 Views)

Background: Premature chromosome condensation (PCC) is a method for interphase chromosome analysis in biodosimetry. This study was performed to verify the usefulness of PCC induced by calyculin A in human peripheral blood lymphocytes (pbl) for biological dosimetry and possible construction of dose-response curve.

Materials and Methods: Peripheral blood was obtained from a healthy donor and exposed to various doses (0.25–4 Gy) of γ -rays. The frequency of simple breaks and dicentrics were scored in G2/M chromosomes of Giemsa stained cells.

Results: Results show that the frequency of simple chromosome breaks appears to increase linearly with dose while the frequency of dicentrics apparently increases linear-quadratically with the dose.

Conclusion: Induction of chromosome condensation by calyculin A is a powerful biodisimetric method, which provides a high number of spreads for analysis. With the use of this method, it is possible to overcome problems related to low mitotic index or cell-cycle alterations in routine metaphase analysis and low fusion rate in conventional PCC technique. Iran . J. Radiat. Res., 2004 2 (1): 15-20

Keywords: PCC, calyculin A, human lymphocytes
Full-Text [PDF 129 kb]   (3728 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Add your comments about this article
Your username or Email:


XML     Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

S. Hosseini, H. Mozdarani. Induction of premature chromosome condensation (PCC) by calyculin A for biodosimetry. Int J Radiat Res. 2004; 2 (1) :15-20
URL: http://ijrr.com/article-1-36-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 2, Issue 1 (6-2004) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.04 seconds with 32 queries by YEKTAWEB 4419