[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 6, Issue 4 (3-2009) ::
Int J Radiat Res 2009, 6(4): 195-200 Back to browse issues page
Preparation and quality control of radiolabeled streptokinase for Thrombosis imaging
A.R. Jalilian Dr. , F. Mirazizi, H. Nazem, J. Garousi, F. Bolourinovin, H. Sadeghpour
, ajalilian@nrcam.org
Abstract:   (12087 Views)
Background: In order to diagnose the site of thrombi, radiolabeled streptokinase can be prepared. The radiolabeled compound can be used in imaging of thrombi in many cardiovascular diseases. Materials and Methods: Streptokinase was successively labeled with [67Ga]-gallium chloride using cyclic DTPA-dianhydride. The conjugation with DTPA was optimized for concentration, time and temperature followed by size exclusion chromatography using G-50 Sephadex. The radiochemical purity of the tracer was checked using HPLC and ITLC methods. The biodistribution studies were performed in normal rats up to 167 h using tissue counting and preliminary SPECT studies up to 2 h. Results: The radiolabeled enzyme was prepared in 60 minutes after incubation at room temperature, with the radiochemical purity of >95% (HPLC) and >99% (ITLC) methods. The radioactivity was accumulated in lung, intestine and liver as shown by scarification and SPECT (Single Photon Emission Computed Tomography) methods. Conclusion: Radiolabeled Streptokinase was prepared in suitable radiochemical purity and its biodistribution is comparable to other radiolabeled proteins. Further studies are required to investigate the imaging properties of the tracer in appropriate animal model. Iran. J. Radiat. Res., 2009 6 (4): 195-200
Keywords: Streptokinase, gallium-67, thrombosis, SPECT, radiopharmaceuticals.
Full-Text [PDF 413 kb]   (1894 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Jalilian A, Mirazizi F, Nazem H, Garousi J, Bolourinovin F, Sadeghpour H. Preparation and quality control of radiolabeled streptokinase for Thrombosis imaging. Int J Radiat Res. 2009; 6 (4) :195-200
URL: http://ijrr.com/article-1-492-en.html


Volume 6, Issue 4 (3-2009) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.05 seconds with 33 queries by YEKTAWEB 3877