[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
For Authors::
For Reviewers::
Subscription::
News & Events::
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE

:: Volume 21, Issue 3 (6-2023) ::
Int J Radiat Res 2023, 21(3): 601-604 Back to browse issues page
Micronucleus assay in the blood of residents in Mamuju, West Sulawesi, Indonesia, a high background radiation area
N. Khoirunnisa , S. Purnami , D. Tetriana , D. Dasumiati , D. Ramadhani , H.N.E. Surniyantoro , M. Syaifudin
Research Center for Radioisotopes, Radiopharmaceuticals and Biodosimetry Technology, Research Organization for Nuclear Energy (ORTN), National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten, Indonesia , harr005@brin.go.id
Abstract:   (1116 Views)
Background: Mamuju is regarded as a high background radiation location (HBRA). The local population's cells may be harmed by the high ionizing radiation dosage in this region. Micronuclei (MN), nucleoplasmic bridges (NPB), nuclear buds (NBUD), and 8-shaped blood cells can be used to detect cytogenetic abnormalities. By comparing blood samples from HBRA to control site samples, this study focused on determining the impacts of high-risk levels of natural radiation in the cells. Material and Methods: The study employed a cytokinesis-block micronucleus cytome (CBMN Cyt) test. Results: In comparison to the control group, the study group had a higher nuclear division index (NDI) and a higher frequency of all abnormalities. The formation of MN and 8-shaped nuclei was significantly influenced by high natural radiation, whereas levels of NPB and NBUD were not significantly affected. Cytogenetic abnormalities were within normal limits and did not correlate with age or gender. Conclusion: Chronic high background radiation exposure impacted human lymphocyte cell growth and the levels of certain biomarkers.
Keywords: CBMN cyt assay, cytogenetic abnormalities, high background radiation area, Mamuju residents
Full-Text [PDF 547 kb]   (572 Downloads)    
Type of Study: Short Report | Subject: Radiation Biology
References
1. Ayua TJ, Tyovenda AA, Igyuse IS, Ejegwoya OP (2017) Determination of ionizing radiation exposure levels within four local mining sites selected from Sardauna Local Government Area of Taraba State - Nigeria. Int J Phys, 5(5): 157-161. [DOI:10.12691/ijp-5-5-3]
2. Aliyu AS and Ramli AT (2015) The world's high background natural radiation areas (HBNRAs) revisited: A broad overview of the dosimetric, epidemiological and radiobiological issues. Radiat Measur, 73: 51-59. [DOI:10.1016/j.radmeas.2015.01.007]
3. Vaiserman A, Koliada A, Zabuga O, Socol Y (2018) Health impacts of low-dose ionizing radiation: current scientific debates and regulatory issues. Dose Response, 16(3): 1-27. [DOI:10.1177/1559325818796331] [PMID] []
4. Syaeful H, Sukadana IG, Sumaryanto A (2014) Radiometric mapping for naturally occurring radioactive materials (NORM) assessment in Mamuju, West Sulawesi. Atom Indonesia, 40(1):33-39. [DOI:10.17146/aij.2014.263]
5. Nugraha ED, Hosoda M, Kusdiana K, et al. (2021) Comprehensive exposure assessments from the viewpoint of health in a unique high natural background radiation area, Mamuju, Indonesia. Sci Rep, 11: 1-16. [DOI:10.1038/s41598-021-93983-2] [PMID] []
6. Kiang JG and Olabisi AO (2019) Radiation: a poly-traumatic hit leading to multi-organ injury. Cell Biosci, 9: 1-15. [DOI:10.1186/s13578-019-0286-y] [PMID] []
7. Fenech M (2007) Cytokinesis-block micronucleus assay evolves into a "cytome" assay of chromosomal instability, mitotic dysfunction, and cell death. Mutat Res, 600: 58-66. [DOI:10.1016/j.mrfmmm.2006.05.028] [PMID]
8. Tian XL, Lu X, Cai TJ, Lyu YM, Tian M, Liu QJ (2021) Cytogenetic monitoring of peripheral blood lymphocytes from medical radiation professionals occupationally exposed to low-dose ionizing radiation. Mutat Res Genet Toxicol Environ Mutagen, 867: 503370. [DOI:10.1016/j.mrgentox.2021.503370] [PMID]
9. Torres-Bugarin O, Covarrubias-Bugarin R, Zamora-Perez AL, et al. (2007) Anabolic androgenic steroids induce micronuclei in buccal mucosa cells of bodybuilders. Br J Sports Med, 41(9): 592-596. [DOI:10.1136/bjsm.2006.032474] [PMID] []
10. Ramadhani D, Sardini S, Lubis M, Syaifudin M (2016) Evaluation of lymphocytes proliferation in Botteng village (a high background radiation area) inhabitants using binucleate index. J Appl Isot Radiat, 12(2): 107-112. [DOI:10.17146/jair.2016.12.2.3564]
11. Kravtsov V, Livanova A, Starkova Y (2017) Nuclear abnormalities of lymphocytes as the simplest markers for bioindication test in case of mass casualty events involving radiation exposure. Emerg Med (Los Angel), 7(4): 1-6. [DOI:10.4172/2165-7548.1000356]
12. Wodarz D, Sorace R, Komarova NL (2014) Dynamics of cellular responses to radiation. PloS Comput Biol, 10(4): 1-11. [DOI:10.1371/journal.pcbi.1003513] [PMID] []
13. Purnami S, Lubis M, Suryadi, Syaifudin M (2020) The assessment of mitotic and nuclear division indexes as biomarkers for estimating the risk on the health of residents exposed to the high natural radiation of Mamuju, West Sulawesi. J Phys: Conf Ser, 1436: 1-8. [DOI:10.1088/1742-6596/1436/1/012032]
14. Sinitsky MY and Druzhinin VG (2014) The application of the cytokinesis-block micronucleus assay on peripheral blood lymphocytes for the assessment of genome damage in long-term residents of areas with high radon concentration. J Radiat Study, 55(1): 61-66. [DOI:10.1093/jrr/rrt091] [PMID] []
15. Syaifudin M, Ramadhani D, Purnami S, et al. (2022) The evaluation of frequencies of cytogenetic biomarkers in lymphocyte of residents from high natural radiation area in West Sulawesi, Indonesia. Int J Adv Sci Eng Inf Technol, 12(1): 263-269. [DOI:10.18517/ijaseit.12.1.12665]
16. Lindberg HK, Wang X, Jarventaus H, et al. (2007) Origin of nuclear buds and micronuclei in normal and folate-deprived human lymphocytes. Mutat Res, 617: 33-45. [DOI:10.1016/j.mrfmmm.2006.12.002] [PMID]
17. Mortazavi S, Mosleh-Shirazi M, Tavassoli A, et al. (2011) A comparative study on the increased radioresistance to lethal doses of gamma rays after exposure to microwave radiation and oral intake of flaxseed oil. Int J Radiat Res, 9: 9-14.
18. Syaifudin M, Defiyandra VP, Nurhayati S, et al. (2018) Micronucleus assay-based evaluation of radiosensitivity of lymphocytes among inhabitants living in high background radiation area of Mamuju, West Sulawesi, Indonesia. Genome Integr, 9(2): 1-5. [DOI:10.4103/genint.genint_2_18] [PMID] []
19. Karuppasamy CV, Ramachandran EN, Kumar VA, et al. (2016) Peripheral blood lymphocyte micronucleus frequencies in men from areas of Kerala, India, with high vs. normal levels of natural background ionizing radiation. Mutat Res Genet Toxicol Environ Mutagen, 800: 40-45. [DOI:10.1016/j.mrgentox.2016.03.005] [PMID]
20. Salimi M and Mozdarani H (2015) Different aspects of cytochalasin B Blocked micronucleus cytome (CBMN cyt) assay as a comprehensive measurement tool for radiobiological studies, biological dosimetry, and genome instability. Int J Radiat Res, 13(2): 100-126.
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khoirunnisa N, Purnami S, Tetriana D, Dasumiati D, Ramadhani D, Surniyantoro H et al . Micronucleus assay in the blood of residents in Mamuju, West Sulawesi, Indonesia, a high background radiation area. Int J Radiat Res 2023; 21 (3) :601-604
URL: http://ijrr.com/article-1-4923-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 3 (6-2023) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.05 seconds with 50 queries by YEKTAWEB 4660