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:: Volume 21, Issue 4 (10-2023) ::
Int J Radiat Res 2023, 21(4): 609-614 Back to browse issues page
Fractionated radiation exposure enhances the DNA repair capacity to acquire radioresistance in HCT8 human colorectal cancer cells
K. Huang , M. Omura , C. Yan , L. Abdelghany , X. Zhang , T-S. Li
Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan , litaoshe@nagasaki-u.ac.jp
Abstract:   (872 Views)
Background: Fractionated radiotherapy is widely used for cancer treatment because of its advantages in the preservation of normal tissues; however, it may amplify the radioresistance of cancer cells. In this study, we aimed to understand whether and how fractionated radiation exposure induces radioresistance. Materials and Methods: HCT8 human colorectal cancer cells received a total X-ray dose of 5 Gy in either a single treatment (5 Gy administered once) or via fractionated exposure (1 Gy/day treatment for 5 consecutive days). We then examined the radioresistance of cancer cells exposed an additional 2 Gy X-ray by clonogenic assay and Western blot analysis. Results: Cells receiving fractionated exposure showed significantly greater proliferation and clonogenicity than those that received a single dose. Compared with the levels in the intact cells without radiation exposure, the expression levels of γH2AX, phospho-ATM and PARP were significantly enhanced only in the cells exposed to fractionated radiation. In contrast, the expression of cyclin D1 and cyclin E1 was enhanced only in the cells that received a single dose. In addition, the expression of SOD1 and SOD2 was slightly increased in the cells that received either the fractionated exposure or single exposure treatment. Conclusions: Fractionated radiation exposure facilitates radioresistance in HCT8 human colorectal cancer cells predominantly by enhancing their DNA repair capacity.
Keywords: Fractionated radiation exposure, DNA repair, Acquired radioresistance.
Full-Text [PDF 1034 kb]   (575 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
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Huang K, Omura M, Yan C, Abdelghany L, Zhang X, Li T. Fractionated radiation exposure enhances the DNA repair capacity to acquire radioresistance in HCT8 human colorectal cancer cells. Int J Radiat Res 2023; 21 (4) :609-614
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Volume 21, Issue 4 (10-2023) Back to browse issues page
International Journal of Radiation Research
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