[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
For Authors::
For Reviewers::
Subscription::
News & Events::
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE

:: Volume 21, Issue 4 (10-2023) ::
Int J Radiat Res 2023, 21(4): 615-619 Back to browse issues page
Effect of probiotics and melatonin on inflammatory cytokines and oxidative stress status in distant organs after local radiotherapy: An experimental study
C. Şahin , S. Aras , S. Mirapoğlu , M. Arpacık , C. Gül , B.N. Ozkan , H. Beyaztaş , E.M. Güler , A. Kaymakcı
University of Health Sciences Umraniye Training and Research Hospital, Department of Pediatric Surgery, Istanbul, Turkey , ceyhan.sahin60@gmail.com
Abstract:   (960 Views)
Background: Abdominopelvic radiotherapy (RT) can lead to inflammatory and oxidative changes in non-targeted organs, such as the lungs. This study aimed to investigate the protective effect of probiotic and melatonin administration on the inflammatory and oxidative changes in the lungs after abdominopelvic RT in Wistar albino rats. Materials and Methods: Thirty female Wistar albino rats were divided into four groups: Group 1 (Control), Group 2 (RT) receiving a single dose of radiotherapy to the abdominopelvic region, Group 3 (RT + Melatonin) receiving a single dose of melatonin intraperitoneally before radiotherapy, and Group 4 (RT + Probiotic) receiving probiotic containing Lactobacillus rhamnosus GG and BB-12 (1010 CFU) via an orogastric feeding cannula before radiotherapy. After the rats were sacrificed, lung tissue samples were analyzed for the levels of Total Antioxidant Status (TAS), Total Oxidant Status (TOS), Oxidative Stress Index (OSI), Interleukin-1 beta (IL-1β), Interleukin-6 (IL-6), and Tumor Necrosis Factor-alpha (TNF-α). Results: TAS was lower in Group 2 compared to Group 3 or 4 (p<0.001). TOS was higher in Group 2 compared to Group 3 (p=0.007). IL-1β, IL-6, OSI, and TNF-α levels were lower in Group 3 or Group 4 compared to Group 2. Conclusion: Abdominopelvic RT resulted in increased TOS and inflammatory cytokines and decreased TAS in non-targeted lung tissues. However, administration of melatonin or probiotics improved the antioxidant status and mitigated the increase in inflammatory cytokines and OSI caused by RT. Melatonin exhibited more prominent effects.
Keywords: Probiotic, melatonin, lactobacillus, radiotherapy, nontargeted, bystander, lung.
Full-Text [PDF 677 kb]   (484 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
References
1. Venkidesh BS, Shankar SR, Narasimhamurthy RK, et al. (2023) Radioprotective potential of probiotics against gastrointestinal and neuronal toxicity: a preclinical study. Clin Transl Oncol. [DOI:10.1007/s12094-023-03184-8] [PMID] []
2. Yahyapour R, Motevaseli E, Rezaeyan A, et al. (2018) Mechanisms of radiation bystander and non-targeted effects: Implications to Radiation Carcinogenesis and Radiotherapy. Curr Radiopharm, 11(1): 34-45. [DOI:10.2174/1874471011666171229123130] [PMID]
3. Mothersill C and Seymour C (2001) Radiation-induced bystander effects: past history and future directions. Radiat Res, 155(6): 759-67. [DOI:10.1667/0033-7587(2001)155[0759:RIBEPH]2.0.CO;2]
4. Fardid R, Salajegheh A, Mosleh-Shirazi MA, et al. (2023) Melatonin ameliorates the production of COX-2, iNOS, and the formation of 8-OHdG in non-targeted lung tissue after pelvic irradiation. Cell J, 19(2): 324-331.
5. Abadi SH, Shirazi A, Alizadeh AM, et al. (2018) The effect of melatonin on superoxide dismutase and glutathione peroxidase activity, and Malondialdehyde levels in the targeted and the non-targeted lung and heart tissues after irradiation in xenograft mice colon cancer. Curr Mol Pharmacol, 11(4): 326-335. [DOI:10.2174/1874467211666180830150154] [PMID]
6. Khan MA, Hill RP, Van Dyk J (1998) Partial volume rat lung irradiation: an evaluation of early DNA damage. Int J Radiat Oncol Biol Phys, 40(2): 467-76. [DOI:10.1016/S0360-3016(97)00736-0]
7. Khan MA, Van Dyk J, Yeung IW, Hill RP (2003) Partial volume rat lung irradiation; assessment of early DNA damage in different lung regions and effect of radical scavengers. Radiother Oncol, 66(1): 95-102. [DOI:10.1016/S0167-8140(02)00325-0]
8. Ghobadi A, Shirazi A, Najafi M, et al. (2017) Melatonin ameliorates radiation-induced oxidative stress at targeted and nontargeted lung tissue. J Med Phys, 42(4): 241-244. [DOI:10.4103/jmp.JMP_60_17] [PMID] []
9. Najafi M, Shirazi A, Motevaseli E, et al. (2017) Melatonin as an anti-inflammatory agent in radiotherapy. Inflammopharmacology, 25(4): 403-413. [DOI:10.1007/s10787-017-0332-5] [PMID]
10. Shirazi A, Haddadi GH, Asadi-Amoli F, et al. (2011) Radioprotective effect of melatonin in reducing oxidative stress in rat lenses. Cell J, 13(2): 79-82. [DOI:10.1016/S0167-8140(11)71751-0]
11. Shirazi A, Mihandoost E, Mohseni M, et al. (2013) Radio-protective effects of melatonin against irradiation-induced oxidative damage in rat peripheral blood. Phys Med, 29(1): 65-74. [DOI:10.1016/j.ejmp.2011.11.007] [PMID]
12. Mohseni M, Mihandoost E, Shirazi A, et al. (2012) Melatonin may play a role in modulation of bax and bcl-2 expression levels to protect rat peripheral blood lymphocytes from gamma irradiation-induced apoptosis. Mutat Res, 738-739: 19-27. [DOI:10.1016/j.mrfmmm.2012.08.006] [PMID]
13. Hu ZP, Fang XL, Fang N, et al. (2013) Melatonin ameliorates vascular endothelial dysfunction, inflammation, and atherosclerosis by suppressing the TLR4/NF-κB system in high-fat-fed rabbits. J Pineal Res, 55(4): 388-98. [DOI:10.1111/jpi.12085] [PMID]
14. Jang SS, Kim HG, Lee JS (2013) Melatonin reduces X-ray radiation-induced lung injury in mice by modulating oxidative stress and cytokine expression. Int J Radiat Biol, 89(2): 97-105. [DOI:10.3109/09553002.2013.734943] [PMID]
15. Hamade DF, Espinal A, Yu J, et al. (2022) Lactobacillus reuteri releasing IL-22 (LR-IL-22) facilitates intestinal radioprotection for whole-abdomen irradiation (WAI) of ovarian cancer. Radiat Res, 198(1): 89-105. [DOI:10.1667/RADE-21-00224.1] [PMID] []
16. Zhang X, Fisher R, Hou W, et al. (2020) Second-generation probiotics producing IL-22 increase survival of mice after total body irradiation. In Vivo, 34(1): 39-50. [DOI:10.21873/invivo.11743] [PMID] []
17. Lee SU, Jang BS, Na YR, et al. (2023) Effect of Lactobacillus rhamnosus GG for Regulation of Inflammatory Response in Radiation-Induced Enteritis. Probiotics Antimicrob Proteins. [DOI:10.1007/s12602-023-10071-9]
18. Rashed ER, Abdel-Rafei MK, Thabet NM (2021) Roles of simvastatin and sildenafil in modulation of cranial irradiation-induced bystander multiple organs injury in Rats. Inflammation, 44(6): 2554-2579. [DOI:10.1007/s10753-021-01524-w] [PMID]
19. Moon K, Stukenborg GJ, Keim J, Theodorescu D (2006) Cancer incidence after localized therapy for prostate cancer. Cancer, 107(5): 991-8. [DOI:10.1002/cncr.22083] [PMID]
20. Brenner DJ, Curtis RE, Hall EJ, Ron E (2000) Second malignancies in prostate carcinoma patients after radiotherapy compared with surgery. Cancer, 88(2): 398-406. https://doi.org/10.1002/(SICI)1097-0142(20000115)88:2<398::AID-CNCR22>3.0.CO;2-V [DOI:10.1002/(SICI)1097-0142(20000115)88:23.0.CO;2-V]
21. Wang S, Li J, He Y, et al. (2021) Protective effect of melatonin entrapped PLGA nanoparticles on radiation-induced lung injury through the miR-21/TGF-β1/Smad3 pathway. Int J Pharm, 602:120584. [DOI:10.1016/j.ijpharm.2021.120584] [PMID]
22. Wu X, Ji H, Wang Y, et al. (2019) Melatonin alleviates radiation-induced lung injury via regulation of miR-30e/NLRP3 axis. Oxid Med Cell Longev, 10: 4087298. [DOI:10.1155/2019/4087298] [PMID] []
23. Arıcıgil M, Dündar MA, Yücel A, et al. (2017) Melatonin prevents possible radiotherapy-induced thyroid injury. Int J Radiat Biol, 93(12): 1350-1356. [DOI:10.1080/09553002.2017.1397296] [PMID]
24. Tahamtan R, Shabestani Monfared A, et al. (2015) Radioprotective effect of melatonin on radiation-induced lung injury and lipid peroxidation in rats. Cell J, 17(1): 111-20.
25. Dündar G, Günaydın Ö, Yazıcı G, et al. (2023) Investigation of the protective effects of intraperitoneal melatonin in rats receiving laryngeal radiotherapy. Auris Nasus Larynx, 50(3): 395-402. [DOI:10.1016/j.anl.2022.08.003] [PMID]
26. Mansouri-Tehrani HS, Khorasgani MR, Roayaei M (2016) Effects of probiotics with or without honey on radiation-induced diarrhea. Int J Radiat Res, 14(3): 205-213. [DOI:10.18869/acadpub.ijrr.14.3.205]
27. Shi L, Tianqi F, Zhang C, et al. (2023) High-protein compound yogurt with quinoa improved clinical features and metabolism of high-fat diet-induced nonalcoholic fatty liver disease in mice. J Dairy Sci, 106(8): 5309-5327. [DOI:10.3168/jds.2022-23045] [PMID]
28. Zhang L, Kang H, Zhang W, et al. (2015) Probiotics ameliorate benzene-induced systemic inflammation and hematopoietic toxicity by inhibiting Bacteroidaceae-mediated ferroptosis. Sci Total Environ, 899: 165678. [DOI:10.1016/j.scitotenv.2023.165678] [PMID]
29. Olimpio F, da Silva JRM, Vieira RP, et al. (2022) Lacticaseibacillus rhamnosus modulates the inflammatory response and the subsequent lung damage in a murine model of acute lung inflammation. Clinics (Sao Paulo), 77: 100021. [DOI:10.1016/j.clinsp.2022.100021] [PMID] []
30. Garcia-Castillo V, Tomokiyo M, Raya Tonetti F, et al. (2020) Alveolar Macrophages are key players in the modulation of the respiratory antiviral immunity induced by orally administered Lacticaseibacillus rhamnosus CRL1505. Front Immunol, 11: 568636. [DOI:10.3389/fimmu.2020.568636] [PMID] []
31. Joshi N, Walter JM, Misharin AV (2018) Alveolar macrophages. Cell Immunol, 330: 86-90. [DOI:10.1016/j.cellimm.2018.01.005] [PMID]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Şahin C, Aras S, Mirapoğlu S, Arpacık M, Gül C, Ozkan B, et al . Effect of probiotics and melatonin on inflammatory cytokines and oxidative stress status in distant organs after local radiotherapy: An experimental study. Int J Radiat Res 2023; 21 (4) :615-619
URL: http://ijrr.com/article-1-5010-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 21, Issue 4 (10-2023) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.05 seconds with 50 queries by YEKTAWEB 4642