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:: Volume 22, Issue 2 (4-2024) ::
Int J Radiat Res 2024, 22(2): 361-366 Back to browse issues page
MiR-144-3p regulates invasion and proliferation activity in hepatocellular carcinoma by targeting SLITRK4
D.B. Xiao , H. Xu , J.C. Li , K.K. Peng , X.L. Jiang , D.X. Wang , F.F. Zhu
Department of Hepatobiliary and Pancreas Surgery, The First Affiliated Hospital of University of South China, Hengyang City, Hunan Province, 421001, China , zff200078@163.com
Abstract:   (633 Views)
Background: miR-144-3p exerts inhibitory roles in hepatocellular carcinoma (HCC). This paper aims to explore that miR-144-3p and its predictive target gene, SLIT- and NTRK-like family member 4 (SLITRK4), in HCC progression. Materials and Methods: The levels of SLITRK4 and miR-144-3p were determined by western blot and qPCR. A loss- and gain-of-function test was used to test whether the relationship between miR-144-3p and SlitrK4 affected the growth of HCC cells. StarBase examined the miR-144-3p target prediction using SLITRK4. Through the use of a luciferase reporter experiment, miR-144-3p's targeting SLITRK4 was identified. The salvage experiment confirmed that miR-144-3p interacts SLITRK4 to regulate the progression of HCC. Results: Findings showned that the expression of miR-144-3p decreased in HCC tissue and cells. MiR-144-3p inhibits deterioration progression of HCC cell progression. MiR-144-3p targeted SLITRK4 and negatively modulated SLITRK4 levels. Besides, miR-144-3p upregulation decreased HCC migration and invasion activity, while SLITRK4 overexpression reduced this inhibition effects. Conclusions: MiR-144-3p regulates invasion and proliferation activity in HCC by targeting SLITRK4. MiR-144-3P/ SLITRK4 axis might provide a potential anti-cancer therapy pathway for clinical diagnosis, treatment and prognosis in hepatoma.
Keywords: MiR-144-3p, SLITRK4, tumor progression, Hepatocellular carcinoma.
Full-Text [PDF 1227 kb]   (171 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
References
1. 1. Bray F, Ferlay J, Soerjomataram I, et al. (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 68(6): 394-424. [DOI:10.3322/caac.21492]
2. Siegel RL, Miller KD, Jemal A, (2019) Cancer statistics, 2019. CA Cancer J Clin, 69(1): 7-34. [DOI:10.3322/caac.21551]
3. Amini J and Hasanramezani A (2022) AAK1 Circular Regulates Neuronal Development by Interacting with miR-132, miR-146a and miR484. ALKHASS, 4(4): 1-4. [DOI:10.47176/alkhass.4.4.1]
4. Miller KD, Siegel RL, Lin CC, et al. (2016) Cancer treatment and survivorship statistics, 2016. CA Cancer J Clin, 66(4): 271-89. [DOI:10.3322/caac.21349]
5. Sarker D, Plummer R, Meyer T, et al. (2020) MTL-CEBPA, a Small Activating RNA Therapeutic Upregulating C/EBP-α, in Patients with Advanced Liver Cancer: A First-in-Human, Multicenter, Open-Label, Phase I Trial. Clin Cancer Res, 26(15): 3936-3946. [DOI:10.1158/1078-0432.CCR-20-0414]
6. Ambros V (2004) The functions of animal microRNAs. Nature, 431(7006): 350-5. [DOI:10.1038/nature02871]
7. He L and Hannon GJ (2004) MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet, 5(7): 522-31. [DOI:10.1038/nrg1379]
8. Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell, 116(2): 281-97. [DOI:10.1016/S0092-8674(04)00045-5]
9. Kurashige J, Kamohara H, Watanabe M, et al. (2012) MicroRNA-200b regulates cell proliferation, invasion, and migration by directly targeting ZEB2 in gastric carcinoma. Ann Surg Oncol, 19(3): S656-64. [DOI:10.1245/s10434-012-2217-6]
10. Zhang Q, Jin X, Shi W, et al. (2020) A long non-coding RNA LINC00461-dependent mechanism underlying breast cancer invasion and migration via the miR-144-3p/KPNA2 axis. Cancer Cell Int, 20: 137. [DOI:10.1186/s12935-020-01221-y]
11. Sun N, Zhang L, Zhang C, Y Yuan, (2020) miR-144-3p inhibits cell proliferation of colorectal cancer cells by targeting BCL6 via inhibition of Wnt/β-catenin signaling. Cell Mol Biol Lett, 25: 19. [DOI:10.1186/s11658-020-00210-3]
12. Gao ZY, Liu H, Zhang Z (2021) miR-144-3p increases radiosensibility of gastric cancer cells by targeting inhibition of ZEB1. Clin Transl Oncol, 23(3): 491-500. [DOI:10.1007/s12094-020-02436-1]
13. Liu M, Xiao B, Zhu Y, et al. (2023) MicroRNA-144-3p protects against chemotherapy-induced apoptosis of ovarian granulosa cells and activation of primordial follicles by targeting MAP3K9. Eur J Med Res, 28(1): 264. [DOI:10.1186/s40001-023-01231-2]
14. Aruga J and Mikoshiba K (2003) Identification and characterization of Slitrk, a novel neuronal transmembrane protein family controlling neurite outgrowth. Mol Cell Neurosci, 24(1): 117-29. [DOI:10.1016/S1044-7431(03)00129-5]
15. Bertelsen B, Tümer Z, Ravn K (2011) Three new loci for determining x chromosome inactivation patterns. J Mol Diagn, 13(5): 537-40. [DOI:10.1016/j.jmoldx.2011.05.003]
16. Davidson B, Abeler VM, Førsund M, et al. (2014) Gene expression signatures of primary and metastatic uterine leiomyosarcoma. Hum Pathol, 45(4): 691-700. [DOI:10.1016/j.humpath.2013.11.003]
17. Aruga J, Yokota N, Mikoshiba K (2003) Human SLITRK family genes: genomic organization and expression profiling in normal brain and brain tumor tissue. Gene, 315: 87-94. [DOI:10.1016/S0378-1119(03)00715-7]
18. Proenca CC, Gao KP, Shmelkov SV, et al. (2011) Slitrks as emerging candidate genes involved in neuropsychiatric disorders. Trends Neurosci, 34(3): 143-53. [DOI:10.1016/j.tins.2011.01.001]
19. Wu J, Zhao Y, Li F, Qiao B(2019) MiR-144-3p: a novel tumor suppressor targeting MAPK6 in cervical cancer. J Physiol Biochem, 75(2): 143-152. [DOI:10.1007/s13105-019-00681-9]
20. Tian LJ, Wu YP, Wang D, et al. (2019) Upregulation of Long Noncoding RNA (lncRNA) X-Inactive Specific Transcript (XIST) is Associated with Cisplatin Resistance in Non-Small Cell Lung Cancer (NSCLC) by Downregulating MicroRNA-144-3p. Med Sci Monit, 25: 8095-8104. [DOI:10.12659/MSM.916075]
21. Bruix J, Gores GJ, Mazzaferro V(2014) Hepatocellular carcinoma: clinical frontiers and perspectives. Gut, 63(5): 844-55. [DOI:10.1136/gutjnl-2013-306627]
22. Yang H, Zhang L, Wang XD, et al. (2018) Potential targets and clinical value of miR-490-5p in hepatocellular carcinoma: a study based on TCGA, qRT-PCR and bioinformatics analyses. Int J Clin Exp Pathol, 11(3): 1123-1134.
23. Zheng Q, Wei X, Rao J, Zhou C (2020) Identification of key miRNAs in the progression of hepatocellular carcinoma using an integrated bioinformatics approach. PeerJ, 8: e9000. [DOI:10.7717/peerj.9000]
24. Di Palo A, Siniscalchi C, Mosca N, et al. (2020) Proto-oncogene Zbtb7a represses miR-125a-5p transcription in hepatocellular carcinoma cells. Mol Biol Rep, 47(6): 4875-4878. [DOI:10.1007/s11033-020-05512-5]
25. Bashir AO, El-Mesery ME, Anwer R, Eissa LA (2020) Thymoquinone potentiates miR-16 and miR-375 expressions in hepatocellular carcinoma. Life Sci, 254: 117794. [DOI:10.1016/j.lfs.2020.117794]
26. Liang HW, Ye ZH, Yin SY, et al. (2017) A comprehensive insight into the clinicopathologic significance of miR-144-3p in hepatocellular carcinoma. Onco Targets Ther, 10: 3405-3419. [DOI:10.2147/OTT.S138143]
27. Tianhua Y, Dianqiu L, Xuanhe Z, et al. (2020) Long non-coding RNA Sox2 overlapping transcript (SOX2OT) promotes multiple myeloma progression via microRNA-143-3p/c-MET axis. J Cell Mol Med, 24(9): 5185-5194. [DOI:10.1111/jcmm.15171]
28. Gai SY and Yuan ZH (2020) Long non-coding RNA SOX21-AS1 promotes cell proliferation and invasion through upregulating PAK7 expression by sponging miR-144-3p in glioma cells. Neoplasma, 67(2): 333-343. [DOI:10.4149/neo_2020_190509N412]
29. Li X, Li Y, Jiang C, et al. (2020) MicroRNA-144-3p Inhibits Tumorigenesis of Oral Squamous Cell Carcinoma by downregulating ERO1L. J Cancer, 11(3): 759-768. [DOI:10.7150/jca.33267]
30. Wang P, Yang Z, Ye T, et al. (2020) lncTUG1/miR-144-3p affect the radiosensitivity of esophageal squamous cell carcinoma by competitively regulating c-MET. J Exp Clin Cancer Res, 39(1): 7. [DOI:10.1186/s13046-019-1519-y]
31. Wang W, Ge L, Xu XJ, et al. (2019) LncRNA NEAT1 promotes endometrial cancer cell proliferation, migration and invasion by regulating the miR-144-3p/EZH2 axis. Radiol Oncol, 53(4): 434-442. [DOI:10.2478/raon-2019-0051]
32. Song L, Chen L, Luan Q, Kong Q (2019) miR-144-3p facilitates nasopharyngeal carcinoma via crosstalk with PTEN. J Cell Physiol, 234(10): 17912-17924. [DOI:10.1002/jcp.28424]
33. CaoXY, Sun ZY, Zhang LJ, et al. (2019) microRNA-144-3p suppresses human neuroblastoma cell proliferation by targeting HOXA7. Eur Rev Med Pharmacol Sci, 23(2): 716-723.
34. Song L, Peng L Hua, S, et al. (2018) miR-144-5p Enhances the Radiosensitivity of Non-Small-Cell Lung Cancer Cells via Targeting ATF2. Biomed Res Int, 2018: 5109497. [DOI:10.1155/2018/5109497]
35. Liu C, Su C, Chen Y, Li G (2018) MiR-144-3p promotes the tumor growth and metastasis of papillary thyroid carcinoma by targeting paired box gene 8. Cancer Cell Int, 18: 54. [DOI:10.1186/s12935-018-0550-y]
36. Tomita A, Mochizuki H, Tsuboi M, et al. (2019) Development of canine X-chromosome inactivation pattern analysis for the detection of cell clonality by incorporating the examination of the SLIT and NTRK-like family member 4 (SLITRK4) gene. Res Vet Sci, 125: 170-175. [DOI:10.1016/j.rvsc.2019.06.004]
37. Kang H, Han KA, Won SY, et al. (2016) Slitrk missense mutations associated with neuropsychiatric disorders distinctively impair slitrk trafficking and synapse formation. Front Mol Neurosci, 9: 104. [DOI:10.3389/fnmol.2016.00104]
38. Wu J, Zhang T, Chen Y, Ha S (2020) MiR-139-5p influences hepatocellular carcinoma cell invasion and proliferation capacities via decreasing SLITRK4 expression. Biosci Rep, 40(5). [DOI:10.1042/BSR20193295]
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Xiao D, Xu H, Li J, Peng K, Jiang X, Wang D et al . MiR-144-3p regulates invasion and proliferation activity in hepatocellular carcinoma by targeting SLITRK4. Int J Radiat Res 2024; 22 (2) :361-366
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Volume 22, Issue 2 (4-2024) Back to browse issues page
International Journal of Radiation Research
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