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MicroRNA-18a-5p targets Sec61 translocon alpha 1 subunit to repress hepatocellular carcinoma cell growth before chromradiotherapy
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Q. Chen   , M.M. Wang , X-K. Meng , Q.L. Wu |
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Abstract: (205 Views) |
Background: Hepatocellular carcinoma (HCC) cell development was investigated in relation to the regulation of Sec61 translocon alpha 1 subunit (SEC61A1) by microRNA (miR)-18a-5p. Materials and Methods: After collection of clinical samples, the transfection of interfering vectors of miR-18a-5p or SEC61A1 was into HCC cells to figure out their roles in development of HCC. The Pearson test and starBase analyzed the association and target prediction of miR-18a-5p. Subsequently, through the dual luciferase reporter experiment, SEC61A1 can be regulated via miR-18a-5p. The salvage experiment revealed that miR-18a-5p influence the degradation process of Hepatocellular carcinoma through combining SEC61A1. Results: In HCC cells, we found that SEC61A1 was elevated and miR-18a-5p was downregulated. HCC cells deteriorating was considerably slowed down by the enhanced miR-18a-5p level. The outcomes demonstrated that miR-18a-5p can interact and regulate SEC61A1. Additionally, the effects of earlier therapy on HCC cell proliferation can be restored by overexpressing SEC61A1. Conclusion: Overall, in HCC cells and tissues miR-18a-5p was significantly downregulated, and inhibited the proliferative ability of HCC cells by targeting SEC61A1. |
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| Keywords: MicroRNA-18a-5p, Sec61 translocon alpha 1 subunit, hepatocellular carcinoma. |
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Full-Text [PDF 3117 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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