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Multislice CT combined with miR-let-7 for colon cancer diagnosis and miR-let-7 targeting HMGA2 to regulate cell cycle alterations in colon cancer cells
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K. Yang   , Z. Chen |
| Department of General Surgery, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University, Changsha, China , wolf0330cz.edu@hotmail.com |
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Abstract: (40 Views) |
Background: Colon cancer (CC) is one of the most common malignancies in the digestive tract. In this study, we analysed the diagnostic value of multislice CT (MSCT) in combination with miR-let-7 in CC and the mechanism of action of miR-let-7 in CC. Materials and Methods: A total of 108 patients with suspected CC admitted to our hospital between March 2020 and May 2022 were retrospectively analysed; 37 patients were diagnosed with CC, and the remaining 71 were determined to have benign colonic lesions. To compare the diagnostic value of MSCT, miR-let-7, and MSCT combined with miR-let-7 detection for CC, we detected the expression of miR-let-7 and high mobility group AT hook protein 2 (HMGA2) in normal human colonic epithelial FHC cells and human colonic mucosal epithelial HCT116 cells and observed the effect of miR-let-7 on the biological behaviour of CC cells. Results: Compared with the results of pathological biopsy, the diagnostic sensitivity of MSCT combined with miR-let-7 for CC was 97.30%, the specificity was 98.59%, and the accuracy was 98.15%, with a misdiagnosis rate of only 1.85%. After miR-let-7 expression was silenced, the viability of colon cancer cells increased, and the inflammatory response and oxidative stress increased. In addition, there was a targeted regulatory relationship between HMGA2 and miR-let-7, which could reverse the effect of miR-let-7 on colon cancer cells. Conclusion: The combination of MSCT and the miR-let-7 assay is accurate for the diagnosis of CC, and miR-let-7 regulates the biological behaviour of CC cells through HMGA2. |
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| Keywords: Colonic neoplasms, HMGA2 protein, multidetector computed tomography, inflammation, miR-let-7. |
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Full-Text [PDF 1017 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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