|
Enhancement of radiation response by scorpion venom in colorectal cancer cells: An in vitro study
|
N. Chegeni   , R. Moghadas Ghahfarokhi , M. Mahdavinia , A. Karimi , N. Neisi , Z. Salehnia , S. Farhadi Shehni , O. Azadbakht |
| Department of Medical Physics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , omidazadbakht68@gmail.com |
|
|
Abstract: (631 Views) |
Background: Colorectal cancer (CRC) is a highly prevalent malignancy, with conventional treatments often challenged by resistance. Mesobuthus eupeus venom (MEV), recognized for its cytotoxicity against cancer cells, was investigated in this study as a novel adjuvant to CRC radiotherapy, with the hypothesis of enhancing its effectiveness. Materials and Methods: MEV was extracted and fractionated, and its protein content was determined. Human colorectal carcinoma (HT-29) cells were then exposed to varying concentrations of MEV fractions, both alone and combined with X-radiation (2, 4, and 6Gy). Cell viability was assessed using the MTT assay, and clonogenicity was analyzed via clonogenicity assays. Apoptosis induction was analyzed using flow cytometry. Results: MEV significantly reduced HT-29 cell viability in a dose-dependent manner, achieving an IC50 value of 0.2μg/ml at 48 hours. Crucially, combining MEV with radiation led to a marked decrease in colony formation compared to radiation alone. For instance, the combination of MEV with 6Gy radiation resulted in a colony formation rate of 38.9 ± 8.7%, indicating approximately a 30% increase in efficacy compared to radiation alone. Flow cytometry further confirmed a significant increase in apoptosis in CRC cells when MEV was combined with radiation (approximately 48.3% apoptosis in the MEV+2Gy group vs. 27.6% with 2Gy alone). This underscores a synergistic interaction between the two treatments. Conclusion: MEV holds promise as a potential adjuvant to radiotherapy for CRC. The observed synergistic effect suggests MEV could enhance radiotherapy efficacy by sensitizing cancer cells and potentially overcoming treatment resistance. Further investigation into its underlying mechanisms and utility in preclinical and clinical settings is warranted. |
|
| Keywords: Mesobuthus eupeus venom (MEV), colorectal cancer (CRC), radiation, cell viability, apoptosis. |
|
|
Full-Text [PDF 1107 kb]
(221 Downloads)
|
|
Type of Study: Original Research |
Subject:
Radiation Biology
|
|
|
|
|
|
|
| References |
1. 1. El-Qassas J, Abd El-Atti M, El-Badri N (2024) Harnessing the potency of scorpion venom-derived proteins: applications in cancer therapy. Bioresources and Bioprocessing, 11(1): 93. [ DOI:10.1186/s40643-024-00805-0] 2. Biller LH and Schrag D (2021) Diagnosis and treatment of metastatic colorectal cancer: a review. JAMA, 325(7): 669-85. [ DOI:10.1001/jama.2021.0106] 3. Fan A, Wang B, Wang X, Nie Y, Fan D, Zhao X, et al. (2021) Immunotherapy in colorectal cancer: current achievements and future perspective. International Journal of Biological Sciences, 17(14): 3837. [ DOI:10.7150/ijbs.64077] 4. Khosravi M, Mayahi M, Kaviani F, Nemati M (2017) The Effects of Isolated Fractions of Mesobuthus eupeus Scorpion venom on humoral immune response. Journal of Arthropod-Borne Diseases, 11(4): 497. 5. Palma DA, Olson R, Harrow S, Gaede S, Louie AV, Haasbeek C, et al. (2020) Stereotactic ablative radiotherapy for the comprehensive treatment of oligometastatic cancers: long-term results of the SABR-COMET phase II randomized trial. Journal of Clinical Oncology, 38(25): 2830-8. [ DOI:10.1200/JCO.20.00818] 6. Jingu K, Matsushita H, Yamamoto T, Umezawa R, Ishikawa Y, Takahashi N, et al. (2018) Stereotactic radiotherapy for pulmonary oligometastases from colorectal cancer: a systematic review and meta-analysis. Technology in Cancer Research & Treatment, 17: 1533033818794936. [ DOI:10.1177/1533033818794936] 7. Salimi A, Adhami V, Alehashem SHS, Vatanpour H, Sadeghi L (2022) Iranian Mesobuthus eupeus crude venom induces selective toxicity in chronic lymphocytic leukemia B-lymphocytes through lysosomal/mitochondrial dysfunction and reactive oxygen species formation. Asian Pacific Journal of Cancer Prevention, 23(7): 2309. [ DOI:10.31557/APJCP.2022.23.7.2309] 8. Shirmardi SP, Shamsaei M, Gandomkar M, Ghannadi Maragheh M (2011) Synthesis and biodistribution study of a chlorotoxin derivative peptide labeled with 131- iodine for tumor therapy. International Journal of Radiation Research, 8(4): 243-8. 9. Baradaran M, Jalali A, Naderi-Soorki M, Jokar M, Galehdari H (2018) First transcriptome analysis of Iranian scorpion, Mesobuthus eupeus venom gland. Iranian Journal of Pharmaceutical Research, 17(4): 1488. 10. Zargan J, Sajad M, Umar S, Naime M, Ali S, Khan HA (2011) Scorpion (Odontobuthus doriae) venom induces apoptosis and inhibits DNA synthesis in human neuroblastoma cells. Molecular and Cellular Biochemistry, 348: 173-81. [ DOI:10.1007/s11010-010-0652-x] 11. Kuzmenkov AI, Vassilevski AA, Kudryashova KS, Nekrasova OV, Peigneur S, Tytgat J, et al. (2015) Variability of potassium channel blockers in Mesobuthus eupeus scorpion venom with focus on Kv1. 1: An integrated transcriptomic and proteomic study. Journal of Biological Chemistry, 290(19): 12195-209. [ DOI:10.1074/jbc.M115.637611] 12. Sharma A, Baker S, Duijm M, Oomen-de Hoop E, Cornelissen R, Verhoef C, et al. (2020) Prognostic factors for local control and survival for inoperable pulmonary colorectal oligometastases treated with stereotactic body radiotherapy. Radiotherapy and Oncology, 144: 23-9. [ DOI:10.1016/j.radonc.2019.10.004] 13. Valizade M, Vanani AR, Rezaei M, Khorsandi LS, Zeidooni L, Mahdavinia M (2020) Mesobuthus eupeus venom induced injury in the colorectal carcinoma cell line (HT29) through altering the mitochondria membrane stability. Iranian Journal of Basic Medical Sciences, 23(6): 760. 14. Dong W, Wang L, Kong T, He Y (2009) Scorpion venom peptides accelerate hematopoietic recovery of myelosuppression in irradiated mice. The American Journal of Chinese Medicine, 37(04): 701-12. [ DOI:10.1142/S0192415X0900717X] 15. Ahmed LA, Abdou FY, El Fiky AA, Shaaban EA, Ain-Shoka AA (2021) Bradykinin-potentiating activity of a gamma-Irradiated bioactive fraction isolated from scorpion (Leiurus quinquestriatus) venom in rats with doxorubicin-induced acute cardiotoxicity: favorable modulation of oxidative stress and inflammatory, fibrogenic and apoptotic pathways. Cardiovascular Toxicology, 21: 127-41. [ DOI:10.1007/s12012-020-09602-5] 16. Salabi FVBBM (2023) Identification, classification, and characterization of alpha and beta subunits of LVP1 protein from the venom gland of four Iranian scorpion species. Scientific Reports, 13(1): 1-11. [ DOI:10.1038/s41598-023-49556-6] 17. Mirzaei S, Fekri HS, Hashemi F, Hushmandi K, Mohammadinejad R, Ashrafizadeh M, et al. (2021) Venom peptides in cancer therapy: An updated review on cellular and molecular aspects. Pharmacological Research, 164: 105327. [ DOI:10.1016/j.phrs.2020.105327] 18. Shen X, Zhang Y, Dong Y, Qian J (2024) Identification and validation of prognostic models and survival predictions of T cell-inflamed related genes in patients with colorectal cancer. International Journal of Radiation Research, 22(3): 595-602. [ DOI:10.61186/ijrr.22.3.595] 19. Soleymanifard S, Rostamyari M, Jaberi N, Rassouli F, Hashemy S, Mehdizadeh A (2021) Effects of radiation dose on the stemness-related genes expression in colorectal cancer cell line. International Journal of Radiation Research, 19(3): 645-51. [ DOI:10.52547/ijrr.19.3.645] |
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
|
Chegeni N, Moghadas Ghahfarokhi R, Mahdavinia M, Karimi A, Neisi N, Salehnia Z, et al . Enhancement of radiation response by scorpion venom in colorectal cancer cells: An in vitro study. Int J Radiat Res 2026; 24 (2) :423-428 URL: http://ijrr.com/article-1-7022-en.html
|
