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:: Volume 24, Issue 2 (4-2026) ::
Int J Radiat Res 2026, 24(2): 457-563 Back to browse issues page
Effect of conditioned medium from low-dose X-ray irradiated cells on doxorubicin-induced leukemic K562 cells death
S. Wattanapongpitak , S. Kothan , M. Tungjai
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand , mtungjai@gmail.com
Abstract:   (611 Views)
Background: This current determined the effects of conditioned medium from low-dose X-ray irradiated cells on cytotoxicity of doxorubicin (Dox) in leukemic K562 cancer cells. Materials and Methods:  Cells were X-irradiated with 0, 0.02, 0.05, and 0.1 Gy, and then these cells were cultured for 24 h. The culture medium collected from irradiated cells was transferred to non-irradiated cells followed by treatments with 50 and 100 nM of Dox. The biological endpoints, cell viability, lipid peroxidation, intracellular reactive oxygen species (ROS) and intracellular iron, were determined at 48 h after treatment. Results: The results showed that irradiated cells conditioned medium (ICCM) alone significantly decreased in cell viability when compared with control cells. The combination of ICCMs with Dox did not change the cell viability when compared with corresponding controls while it had significant decreases in cell viability when compared with treated cells of 50 and 100 nM Dox alone, respectively. The cell viability effects were used to calculate the synergism quotient values, resulting in values that showed non-synergistic effects in all combination conditions. ICCMs and combination of ICCMs with Dox significantly decreased in lipid peroxidation levels but did not change the intracellular ROS and the intracellular iron levels in K562 cells. Conclusion: These findings revealed that the ICCMs could induce cell death in K562 cells and ICCMs were contributed to the cytotoxicity of Dox in K562 cells. It should be noted that the lipid peroxidation might have involved to ICCMs-induced cell death.
Keywords: X-Rays, leukemia, conditioned culture media.
Full-Text [PDF 985 kb]   (249 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
References
1. 1. Rithidech KN, Golightly M, Whorton E (2008) Analysis of cell cycle in mouse bone marrow cells following acute in vivo exposure to 56Fe ions. J Radiat Res, 49(4):437-43. [DOI:10.1269/jrr.07109]
2. Jelveh S, Kaspler P, Bhogal N, Mahmood J, Lindsay PE, Okunieff P, et al. (2013) Investigations of antioxidant-mediated protection and mitigation of radiation-induced DNA damage and lipid peroxidation in murine skin. Int J Radiat Biol, 89(8): 618-27. [DOI:10.3109/09553002.2013.782450]
3. Villani P, Fresegna AM, Ranaldi R, Eleuteri P, Paris L, Pacchierotti F, et al. (2013) X-ray induced DNA damage and repair in germ cells of PARP1(-/-) male mice. Int J Mol Sci,14(9):18078-92. [DOI:10.3390/ijms140918078]
4. Mozdarani H, Salimi M, Bakhtari N (2017) Inherent radiosensitivity and its impact on breast cancer chemo-radiotherapy. Int J Radiat Res,15(4):325-41.
5. Bond VP, Benary V, Sondhaus CA (1991) A different perception of the linear, nonthreshold hypothesis for low-dose irradiation. Proc Natl Acad Sci USA, 88(19):8666-70. [DOI:10.1073/pnas.88.19.8666]
6. Azzam EI, de Toledo SM, Raaphorst GP, Mitchel RE (1996) Low-dose ionizing radiation decreases the frequency of neoplastic transformation to a level below the spontaneous rate in C3H 10T1/2 cells. Radiat Res,146(4):369-73. [DOI:10.2307/3579298]
7. Wolff S (1998) The adaptive response in radiobiology: evolving insights and implications. Environ Health Perspect, 106 Suppl 1: 277-83. [DOI:10.1289/ehp.98106s1277]
8. Redpath JL, Liang D, Taylor TH, Christie C, Elmore E (2001) The shape of the dose-response curve for radiation-induced neoplastic transformation in vitro: evidence for an adaptive response against neoplastic transformation at low doses of low-LET radiation. Radiat Res,156(6): 700-7. [DOI:10.1667/0033-7587(2001)156[0700:TSOTDR]2.0.CO;2]
9. Feinendegen LE (2005) Evidence for beneficial low level radiation effects and radiation hormesis. Br J Radiol, 78(925): 3-7. [DOI:10.1259/bjr/63353075]
10. Scott BR and Di Palma J (2006) Sparsely ionizing diagnostic and natural background radiations are likely preventing cancer and other genomic-instability-associated diseases. Dose Response, 5(3): 230-55. [DOI:10.2203/dose-response.06-002.Scott]
11. Rithidech KN and Scott BR (2008) Evidence for radiation hormesis after in vitro exposure of human lymphocytes to low doses of ionizing radiation. Dose Response,6(3): 252-71. [DOI:10.2203/dose-response.07-024.Rithidech]
12. Hamada N, Matsumoto H, Hara T, Kobayashi Y (2007) Intercellular and intracellular signaling pathways mediating ionizing radiation-induced bystander effects. J Radiat Res, 48(2): 87-95. [DOI:10.1269/jrr.06084]
13. Huang YH, Yang PM, Chuah QY, Lee YJ, Hsieh YF, Peng CW, et al. (2014) Autophagy promotes radiation-induced senescence but inhibits bystander effects in human breast cancer cells. Autophagy,10(7): 1212-28. [DOI:10.4161/auto.28772]
14. Chen ZY, Hu YY, Hu XF, Cheng LX (2018) The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells. J Radiat Res, 59(5): 555-64. [DOI:10.1093/jrr/rry048]
15. Azzam EI, de Toledo SM, Little JB (2003) Oxidative metabolism, gap junctions and the ionizing radiation-induced bystander effect. Oncogene, 22(45): 7050-7. [DOI:10.1038/sj.onc.1206961]
16. Mahmoudi F, Shahbazi-Gahrouei D, Chegeni N, Saeb M, Sadeghi V, Hemati S (2022) Potential implications of the radiation-induced bystander effect for spatially fractionated radiotherapy: A theoretical simulation study. Int J Radiat Res, 20(3): 657-64.
17. Ramadan LM and Abdelrazzak AB (2024) The non-targeted effect increases the risk of the radiation-induced myocardial injury. Int J Radiat Res, 22(2): 289-95. [DOI:10.61186/ijrr.22.2.289]
18. Rezaei M, Kamran Samani R, Kazemi M, Shanei A, Hejazi SH (2021) Induction of a bystander effect after therapeutic ultrasound exposure in human melanoma: In-vitro assay. Int J Radiat Res,19(1): 183-9. [DOI:10.29252/ijrr.19.1.183]
19. Shirani S, Mozdarani H, Mahmoodzadeh A, Salimi M (2015) Radio-adaptive response of peripheral blood lymphocytes following bystander effects induced by preirradiated CHO-K1 cells using the micronucleus assay. Int J Radiat Res,13(2):151-6.
20. Bahreyni Toossi MT, Khademi S, Azimian H, Mohebbi S, Soleymanifard S (2017) Assessment of the dose-response relationship of radiation-induced bystander effect in two cell lines exposed to high doses of ionizing radiation (6 and 8 Gy). Cell J, 19(3): 434-42.
21. Faqihi F, Neshastehriz A, Soleymanifard S, Shabani R, Eivazzadeh N (2015) Radiation-induced bystander effect in non-irradiated glioblastoma spheroid cells. J Radiat Res, 56(5): 777-83. [DOI:10.1093/jrr/rrv039]
22. Tungjai M, Phathakanon N, Rithidech KN (2017) Effects of medical diagnostic low-dose X rays on human lymphocytes: Mitochondrial membrane potential, apoptosis and cell cycle. Health Phys, 112(5): 458-64. [DOI:10.1097/HP.0000000000000647]
23. Tungjai M, Phathakanon N, Ketnuam P, Tinlapat J, Kothan S (2018) Determination of hemolysis, osmotic fragility and fluorescence anisotropy on irradiated red blood cells as a function of kV of medical diagnostic X-rays. Int J Radiat Res,16(1): 123-7.
24. Tungjai M, Sopapang J, Tasri N, Osothsongkroh C, Jantarato A, Kothan S (2019) The effects of medical diagnostic low dose X-rays after in-vitro exposure of human red blood cells: Hemolysis and osmotic fragility. ToxEHS, 11(3): 237-43. [DOI:10.1007/s13530-019-0409-1]
25. Supawat B, Homnuan P, Kanthawong N, Semrasa N, Tima S, Kothan S, et al. (2021) Different responses of normal cells (red blood cells) and cancer cells (K562 and K562/Dox cells) to low-dose 137Cs gamma‑rays. Mol Clin Oncol, 14(4): 74. [DOI:10.3892/mco.2021.2236]
26. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. (2021) Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 71(3): 209-49. [DOI:10.3322/caac.21660]
27. Zhong S, Chen Z, Yu X, Chen W, Lv M, Ma T, et al. (2014) Tea consumption and leukemia risk: a meta-analysis. Tumour Biol, 35(6): 5205-12. [DOI:10.1007/s13277-014-1675-9]
28. Mahbub AA, Le Maitre CL, Haywood-Small SL, McDougall GJ, Cross NA, Jordan-Mahy N (2013) Differential effects of polyphenols on proliferation and apoptosis in human myeloid and lymphoid leukemia cell lines. Anticancer Agents Med Chem,13(10): 1601-13. [DOI:10.2174/18715206113139990303]
29. Liu X, Ye F, Wu J, How B, Li W, Zhang DY (2015) Signaling proteins and pathways affected by flavonoids in leukemia cells. Nutr Cancer,67(2): 238-49. [DOI:10.1080/01635581.2015.989372]
30. Davenport A, Frezza M, Shen M, Ge Y, Huo C, Chan TH, et al. (2010) Celastrol and an EGCG pro-drug exhibit potent chemosensitizing activity in human leukemia cells. Int J Mol Med, 25(3): 465-70. [DOI:10.3892/ijmm_00000366]
31. Gewirtz DA (1999) A critical evaluation of the mechanisms of action proposed for the antitumor effects of the anthracycline antibiotics adriamycin and daunorubicin. Biochem Pharmacol, 57(7): 727-41. [DOI:10.1016/S0006-2952(98)00307-4]
32. Minotti G, Menna P, Salvatorelli E, Cairo G, Gianni L (2004) Anthracyclines: molecular advances and pharmacologic developments in antitumor activity and cardiotoxicity. Pharmacol Rev, 56(2):185-229. [DOI:10.1124/pr.56.2.6]
33. Meredith AM and Dass CR (2016) Increasing role of the cancer chemotherapeutic doxorubicin in cellular metabolism. J Pharm Pharmacol, 68(6):729-41. [DOI:10.1111/jphp.12539]
34. Supawat B, Moungthong P, Chanloi C, Jindachai N, Tima S, Kothan S, et al. (2020) Effects of gadolinium-based magnetic resonance imaging contrast media on red blood cells and K562 cancer cells. J Trace Elem Med Biol, 62:126640. [DOI:10.1016/j.jtemb.2020.126640]
35. Arbab IA, Abdul AB, Sukari MA, Abdullah R, Syam S, Kamalidehghan B, et al. (2013) Dentatin isolated from Clausena excavata induces apoptosis in MCF-7 cells through the intrinsic pathway with involvement of NF-κB signalling and G0/G1 cell cycle arrest: a bioassay-guided approach. J Ethnopharmacol, 145(1): 343-54. [DOI:10.1016/j.jep.2012.11.020]
36. Kntayya SB, Ibrahim MD, Mohd Ain N, Iori R, Ioannides C, Abdull Razis AF (2018) Induction of apoptosis and cytotoxicity by isothiocyanate sulforaphene in human hepatocarcinoma HepG2 cells. Nutrients, 10(6). [DOI:10.3390/nu10060718]
37. Aye KT, Wattanapongpitak S, Supawat B, Kothan S, Udomtanakunchai C, Tima S, et al. (2021) Gallic acid enhances pirarubicin‑induced anticancer in living K562 and K562/Dox leukemia cancer cells through cellular energetic state impairment and P-glycoprotein inhibition. Oncol Rep, 46(4): 1-10. [DOI:10.3892/or.2021.8178]
38. Supawat B, Udomtanakunchai C, Kothan S, Tungjai M (2019) The effects of iodinated radiographic contrast media on multidrug-resistant K562/Dox cells: Mitochondria impairment and P-glycoprotein inhibition. Cell Biochem Biophys, 77(2): 157-63. [DOI:10.1007/s12013-019-00868-3]
39. Loetchutinat C, Kothan S, Dechsupa S, Meesungnoen J, Jay-Gerin J-P, Mankhetkorn S (2005) Spectrofluorometric determination of intracellular levels of reactive oxygen species in drug-sensitive and drug-resistant cancer cells using the 2′,7′-dichlorofluorescein diacetate assay. Radiat Phys Chem,72: 323-31. [DOI:10.1016/j.radphyschem.2004.06.011]
40. Herok R, Konopacka M, Polanska J, Swierniak A, Rogolinski J, Jaksik R, et al. (2010) Bystander effects induced by medium from irradiated cells: similar transcriptome responses in irradiated and bystander K562 cells. Int J Radiat Oncol Biol Phys, 77(1): 244-52. [DOI:10.1016/j.ijrobp.2009.11.033]
41. Ayala A, Muñoz MF, Argüelles S (2014) Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev, 2014: 360438. [DOI:10.1155/2014/360438]
42. Yin H, Xu L, Porter NA (2011) Free radical lipid peroxidation: mechanisms and analysis. Chem Rev, 111(10): 5944-72. [DOI:10.1021/cr200084z]
43. Sokolov MV and Neumann RD (2010) Radiation-induced bystander effects in cultured human stem cells. PLoS One, 5(12): e14195. [DOI:10.1371/journal.pone.0014195]
44. Soleymanifard S, Bahreyni Toossi MT, Sazgarnia A, Mohebbi S (2013) The role of target and bystander cells in dose-response relationship of radiation-induced bystander effects in two cell lines. Iran J Basic Med Sci, 16(2): 177-83.
45. Soleymanifard S, Toossi MT, Samani RK, Mohebbi S (2014) Investigation of the bystander effect in MRC5 cells after acute and fractionated irradiation in vitro. J Med Phys, 39(2): 93-7. [DOI:10.4103/0971-6203.131282]
46. Jabbari N, Nawaz M, Rezaie J (2019) Bystander effects of ionizing radiation: conditioned media from X-ray irradiated MCF-7 cells increases the angiogenic ability of endothelial cells. Cell Commun Signal, 17(1): 165. [DOI:10.1186/s12964-019-0474-8]
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Wattanapongpitak S, Kothan S, Tungjai M. Effect of conditioned medium from low-dose X-ray irradiated cells on doxorubicin-induced leukemic K562 cells death. Int J Radiat Res 2026; 24 (2) :457-563
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Volume 24, Issue 2 (4-2026) Back to browse issues page
International Journal of Radiation Research
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