|
Picosecond UV laser induced morphological, biochemical and biological changes in Bombyx mori
|
S.R. Hosagoudar   , H.B. Manjunatha |
| , satya_h2001@yahoo.com |
|
|
Abstract: (11621 Views) |
| Background: In the light of various applications
of UV laser in biological system, we have investigated
the effect of picosecond UV laser radiation on
silkworm Bombyx mori. Materials and Methods: The
eggs of NB4D2 of different stages were exposed to
pico second pulse laser at 355 nm from Nd:YAG laser
for different durations. Results: Due to irradiation
alterations in crescent larval body markings, pupae
with transpositioned antennae, pseudo abdominal
and caudal legs were produced from 2 and 8 hr old
embryos irradiated for 30 and 50 seconds
respectively. Moths devoid of antennae and underdeveloped
legs were also produced from 16 and 8 hr
old embryos irradiated for 60 and 50 seconds respectively.
The morphological anomalies were found
highest in picosecond (6.16%) compare to
nanosecond (1%) irradiated embryos at 8 and 16 hrs
respectively and it is duration dependent. SDS-PAGE
analysis of embryo revealed the occurrence of a 41
kDa new protein and delayed utilization of yolk
proteins in the irradiated embryos. The larval haemolymph
protein profile also exhibited 24, 25 and 6.2
kDa new protein bands. Embryo hatching, larval
weight and cocooning rate was significantly affected
and declined as duration of irradiation increases.
Conclusion: It is clear from the present study that
morphological anomalies and distinct variations in
egg and haemolymph proteins establish a strong
evidence that UV picosecond laser not only cause
damage on embryonic cells but also interfere in
transcriptional factors encode for organogenesis and
proteins. Thus present study envisage the use of UV
laser irradiation as a potential tool in investigating the
embryonic and postembryonic development and
cross-linking between DNA and protein using
silkworm B. mori as molecular model. Iran. J. Radiat.
Res., 2011 9(2): 127-137 |
|
| Keywords: Laser, UV, silkworm, radiation, Bombyx mor |
|
|
Full-Text [PDF 301 kb]
(2791 Downloads)
|
|
Type of Study: Original Research |
Subject:
Radiation Biology
|
|
|
|
|
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
