:: Volume 17, Issue 1 (1-2019) ::
Int J Radiat Res 2019, 17(1): 47-55 Back to browse issues page
Induction of radioresistant nasopharyngeal carcinoma cell line CNE-2R by repeated high-dose X-ray irradiation
D. Zhu, M. Huang, M. Fang, A. Li, Z. Liu, M. Shao, Y. Liu, J. Yang, Q. Fan PhD
School of Traditional Chinese Medicine, Southern Medical University, Guangdong Guangzhou, 510515, China 2NanFang Hospital, Guangdong Guangzhou, 510515, China , fanqinshimen@yahoo.com
Abstract:   (1755 Views)
Background: All To enhance the curative effect of radiotherapy, we established a radio-resistant cell line, CNE-2R from CNE-2, a radio-sensitive type of CNE, through repeated irradiation. The developed cell line provides a basis for further studies on the radio-resistance of CNE and the molecular mechanism of radiotherapy sensitization drugs. Materials and Methods: The CNE-2 cell line was selected and exposed repeatedly to high-dose X-ray intermittent irradiation (6 Gy/fraction for seven fractions and 20 Gy/fraction for three fractions). After the entire irradiation process, 20 Gy/time was administered to CNE irregularly. The cell growth curves of CNE-2 and CNE-2R were constructed based on MTT assays. Dose-survival curves were obtained through colony-forming tests and subjected to linear quadratic formulation matching. SF2 and correlation parameters of radiation biology were calculated. Changes in the cell cycle of CNE-2 and CNE-2R were also assessed by serum starvation. Results: The doubling time of CNE-2 was 2.4 days, which is 0.4 days shorter than that of CNE-2R, indicating the faster growth rate of CNE-2. In the dose-survival equation of the survival clone test, the sensitization ratio of CNE-2R was enhanced relative to that of CNE-2. After synchronization and desynchronization for 24 h, G1 was arrested in CNE-2R. In the S phase, which is insensitive to radiation, the ratio of G1 increased, hence altering the cell cycle. Conclusion: We have established a radio-resistant nasopharyngeal carcinoma cell line by repeated exposure to radiation which is relevant to changes in the cell cycle.
Keywords: Cell cycle, cell growth curve, cell radiation biology parameter, nasopharyngeal carcinoma, radio-resistance.
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Type of Study: Original Research | Subject: Radiation Biology

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