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:: Volume 12, Issue 4 (10-2014) ::
Int J Radiat Res 2014, 12(4): 377-382 Back to browse issues page
Comparison between Amperometric and Chemiluminescence methods in detection and dosimetry of cobalt-60 gamma ray emission
M. Shourian, H. Ghourchian
Institute of Biochemistry and Biophysics, University of Tehran, P.O. Box 13145-1384, Tehran, Iran , hadi@ibb.ut.ac.ir
Abstract:   (5777 Views)

Background: Hydrogen peroxide (H2O2), produced by gamma ray irradiation to watery solution, was used as an analytical parameter for dosimetry of cobalt-60 (60Co) radiation. Materials and Methods: Detection of the produced H2O2 was carried out using two methods: an amperometric biosensor fabricated by immobilization of anthraquinone 2-carboxylic acid modified horseradish peroxidase on glassy carbon electrode, and chemiluminescence technique using luminol as a fluorophore and diperiodatocuprate as a catalyst. Results: In the first method, at the applied potential of -550 mV (vs. Ag/AgCl) the biosensor showed the sensitivity of 74.46 nA/Gray and detection limit of 0.061 Gray (R.S.D=3.67% for N=3) towards H2O2 produced by 60Co. In the second method, the sensitivity and detection limit for 60Co gamma ray was determined to be 2.34 (luminescence intensity/Gray) and 0.069 Gray (R.S.D=4.16% for N=3), respectively. Conclusion: The obtained results revealed that both proposed techniques, with leaner range from 0.25 to 5 Grays are applicable for detection and dosimetry of gamma ray from 60Co.

Keywords: Cobalt-60, gamma ray, hydrogen peroxide, electrochemistry, chemiluminescence
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Type of Study: Short Report | Subject: Radiation Biology
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Shourian M, Ghourchian H. Comparison between Amperometric and Chemiluminescence methods in detection and dosimetry of cobalt-60 gamma ray emission . Int J Radiat Res. 2014; 12 (4) :377-382
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Volume 12, Issue 4 (10-2014) Back to browse issues page
International Journal of Radiation Research
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