[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
IJRR Information::
For Authors::
For Reviewers::
Subscription::
News & Events::
Web Mail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
ISSN
Hard Copy 2322-3243
Online 2345-4229
..
Online Submission
Now you can send your articles to IJRR office using the article submission system.
..

AWT IMAGE

AWT IMAGE

:: Volume 19, Issue 1 (1-2021) ::
Int J Radiat Res 2021, 19(1): 99-111 Back to browse issues page
Ionizing radiation induces ferroptosis in splenic lymphocytes of mice
X. Zhang , H. Liu , X. Xing , M. Tian , X. Hu , F. Liu , J. Feng , S. Chang , P. Liu , H. Zhang
Department of Nuclear Science and Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, Jiangsu, P.R. China , zhangxiaohong@nuaa.edu.cn
Abstract:   (2433 Views)
Backgrounds: It remains unclear whether radiation-induced haemorrhage in the spleen causes iron accumulation, and subsequently, ferroptosis in splenic lymphocytes. In this study, we investigated the occurrence of ferroptosis in splenic lymphocytes of gamma-irradiated mice. Materials and Methods: Mice were subjected to gamma radiation from a 137Cs source. Iron, Ferroportin 1, and iron regulatory protein (IRP) levels in the spleen, and serum iron and hepcidin levels in the blood were measured to study the change in iron metabolism of the irradiated spleen. After Ferrostatin 1/LDN193189 was intraperitoneally injected into mice post-irradiation, the viability of splenic lymphocytes and the splenic index were evaluated to investigate the mechanism of damage induction in splenic lymphocytes. The survival rate of mice was evaluated to identify the radiation mitigator based on the inhibition of ferroptosis. Results: Iron accumulation (up to 0.62 g/g) observed in the spleen of irradiated mice was due to haemorrhage-based haemosiderin. The iron accumulation triggered the IRP-ferroportin 1 axis to increase the level of serum iron to 121.65 mmol/l. LDN193189 was used to demonstrate that the iron accumulation decreased the viability of splenic lymphocytes in irradiated mice, which was subsequently demonstrated to attribute to ferroptosis with the use of Ferrostatin 1 and through detection of ferroptosis-related parameters. The survival rate of irradiated mice was improved upon Ferrostatin-1 (60% with a duration of 120 days) or LDN193189 (40% for the same duration) treatment. Conclusion: Radiation-induced haemorrhage causes ferroptosis in splenic lymphocytes, and anti-ferroptosis is a potential strategy to alleviate immune damage in hematopoietic acute radiation sickness.
Keywords: Hematopoietic acute radiation sickness, iron metabolism, iron accumulation, splenic lymphocyte, ferroptosis.
Full-Text [PDF 2583 kb]   (906 Downloads)    
Type of Study: Original Research | Subject: Medical Physics
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Zhang X, Liu H, Xing X, Tian M, Hu X, Liu F, et al . Ionizing radiation induces ferroptosis in splenic lymphocytes of mice. Int J Radiat Res 2021; 19 (1) :99-111
URL: http://ijrr.com/article-1-3461-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 19, Issue 1 (1-2021) Back to browse issues page
International Journal of Radiation Research
Persian site map - English site map - Created in 0.07 seconds with 50 queries by YEKTAWEB 4645