Background: The purpose of this study was to evaluate the impact of moderate exercise training as an effective strategy to attenuate the harmful effects of electromagnetic radiation emitted from a cell phone on Wistar Rat's semen quality. Materials and Methods: Twenty four male Wistar rats (10 weeks old) were randomly assigned to groups: control group, exercise group, radiation group, and radiation plus exercise group. The animals in radiation and radiation plus exercise groups were exposed to radiofrequency electromagnetic radiation of a cell phone 3 hours/day for 28 days. The animals in exercise and radiation plus exercise groups performed moderate exercise training six days a week for 28 days. At the same time, the control and exercise groups exposed to a mobile phone in switch off. Basic parameters of testes weight, sperm count, motility, progressivity, morphology, and viability assessed. Results: Exposure to the cell phone for 28 days significantly reduced sperm count, progressivity, and normal morphology. Exercise alone caused a significant increase in sperm progressivity only. Radiation plus exercise caused a significant increase in sperm progressivity and morphology compared with the radiation group.