Background: This study aims to explore the therapeutic mechanism of ³²P application combined with timolol maleate for infantile superficial hemangioma. Materials and Methods: In this study, 60 nude mice were enrolled to establish the transplantation model of infantile hemangioma in nude mice. They were randomly divided into control group, administration group and combined administration group, with 20 mice in each group. The expressions of EGFL7 and VEGF were detected by RT-PCR, and the expressions of renin and ang II were detected by Western blot. Results: After the administration of timolol maleate, the expression of EGFL7, VEGF, renin and Ang  II decreased (P < 0.05). The levels of EGFL7, VEGF, renin and Ang II were further down regulated by combined scheme of ³²P application and timolol maleate (P<0.05). Conclusion: ³²P application combined with timolol maleate can improve the therapeutic effect of infantile superficial hemangioma by inhibiting the expression of vascular endothelial growth factor, renin and angiotensin II.