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AWT IMAGE

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:: Volume 22, Issue 1 (1-2024) ::
Int J Radiat Res 2024, 22(1): 131-137 Back to browse issues page
Comparative bio-effect of gadolinium with alternative contrast medium of magnesium chloride and zinc oxide nanoparticles on single-strand DNA following exposure to magnetic resonance imaging
R.M. Shaheen , N.N.A. Bt Nik Ab Razak , M.M. Radhi , N. Binti Suardi , M. Mohammed
School of Physics, Universiti Sains Malaysia, 11800, Gelugor, Pulau Pinang, Malaysia , rmoozshahin@yahoo.com
Abstract:   (360 Views)
Background: Gadolinium (Gd) is a widely used MRI contrast agent that improves visibility and aids in accurate diagnosis. However, conflicting reports exist regarding its genotoxic effects. This study investigates the bio-effects of Gd and alternative contrast media, magnesium chloride (MgCl2), and zinc oxide nanoparticles (ZnO NPs) on DNA single-strand breaks. Materials and Methods: In this in-vitro comparative experiment, 12 adult New Zealand rabbits (males aged between 4.5 to 5.5 months, weighing 2.5 to 3.0 kg) were utilized. The rabbits received intravenous injections of different contrast agents, namely Gd (n=3), MgCl2 (n=3), ZnO NPs (n=3), and a control group (n=3). Following this, all the rabbits were subjected to MRI at 1.5 Tesla (T) with an RF of 64 MHz for 20 minutes. The alkaline comet assay assessed the presence of single-strand breaks (SSB). Results: The results of the study revealed a statistically significant increase in DNA SSB in both the Gd (p<0.010) and ZnO NPs (p=0.006) treated groups compared to the normal control group. However, the alternative MgCl2 treatment did not elicit a statistically significant effect on the DNA single-strand compared to the control group (p=0.277). Conclusion: The contrast medium Gd and alternative ZnO NPs were demonstrated to cause significant DNA single-strand breaks, with the Gd causing more damage than ZnO NPs. However, the alternative contrast MgCl2 was safer with no effect on DNA single-strand. This suggests that MgCl2 is more suitable as an alternative contrast media in MRI scanning.
Keywords: Magnetic resonance imaging, gadolinium, magnesium chloride, zinc oxide nanoparticles, DNA single-strand, alkaline comet assay.
Full-Text [PDF 765 kb]   (167 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
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Shaheen R, Bt Nik Ab Razak N, Radhi M, Binti Suardi N, Mohammed M. Comparative bio-effect of gadolinium with alternative contrast medium of magnesium chloride and zinc oxide nanoparticles on single-strand DNA following exposure to magnetic resonance imaging. Int J Radiat Res 2024; 22 (1) :131-137
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Volume 22, Issue 1 (1-2024) Back to browse issues page
International Journal of Radiation Research
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