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Exosome-delivered miRNA-339 represses the progression of gastric cancer via targeting ZNF689 before chemoradiotherapy
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L. Liang   , Z. Zhang , Z. Cheng , H. Li , H. Jiang |
| Department of Gastrointestinal Surgery, The First Affiliated Hospital of Wannan Medical College, Wuhu, Anhui 241000, China , jianghouxiang2023@163.com |
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Abstract: (428 Views) |
Background: MicroRNAs (miRNAs) have crucial roles in human cancers. Many studies have certified that miRNAs are implicated in tumor progression via exosomes. Nevertheless, whether miRNA-339 is derived by exosomes and its effects in gastric cancer (GC) presents obscure. Therefore, our study attempted to clarify the functional role and molecular mechanism of miRNA-339 in GC. Materials and Methods: In this research, the potential of miRNA-339 in GC was verified through miRNA-339 elevation with cell function assays. Bioinformatics analysis together with mechanical assay was implemented for assessing the regulatory relation between miRNA-339 and zinc finger protein 689 (ZNF689). Moreover, the existence of exosomes was determined via transmission electron microscopy together with nanoparticle tracking analysis. Results: miRNA-339 presented significant down-regulation in GC. miRNA-339 elevation suppressed GC cell proliferation, invasion along with migration while elevated GC cell apoptosis. miRNA-339 targeted ZNF689 3’UTR to repress ZNF689 expression, thereby hindering GC progression. Finally, miRNA-339 was majorly incorporated into exosomes to hinder GC progression. Conclusion: In summary, exosome-delivered miRNA-339 may act to be a tumor repressor in GC by targeting ZNF689, which might be an underlying therapeutic target for GC. |
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| Keywords: Gastric cancer, miRNA-339, ZNF689, exosome. |
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Full-Text [PDF 3711 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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Liang L, Zhang Z, Cheng Z, Li H, Jiang H. Exosome-delivered miRNA-339 represses the progression of gastric cancer via targeting ZNF689 before chemoradiotherapy. Int J Radiat Res 2024; 22 (2) :443-453 URL: http://ijrr.com/article-1-5466-en.html
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