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:: Volume 7, Issue 4 (3-2010) ::
Int J Radiat Res 2010, 7(4): 211-216 Back to browse issues page
Evaluation of combination effects of 2- methoxyestradiol and methoxyamine on IUdRinduced radiosensitization in glioma spheroids
A. Neshasteh-Riz , S. Babaloui, S. Khoei
, neshastehriz@yahoo.com
Abstract:   (14053 Views)
Background: Glioblastoma is the most common and most malignant cancer of central nervous system. Targeted radiotherapy is an effective method toward its treatment. Iododeoxyuridine (IUdR) is a halogenated thymidine analogue known to be effective as a radiosensitizer in human cancer therapy. In this study we have evaluated the combination effects of 2-Methoxyestradiol, an inhibitor of hypoxia inducible factor 1α (HIF-1α) and Methoxyaminem, an inhibitor of base excision repair (BER) pathway on radiosensitization of IUdR in glioblastoma spheroid culture. Materials and Methods: The cytotoxic damages of DNA in U87MG cell line were compared using colony formation assay. Experiments were performed in large spheroids with a diameter of approximately 350μm. Results: Evaluation of the effects of IUdR with 2ME2 and MX pretreatment on spheroid cultured cell followed by ionizing irradiation showed more enhancemented (p≤0.001) IUdR induced-radiosensitization. These results introduced a key role for 2ME2 in IUdR related studies. Conclusion: Pretreatment of tumor cells with IUdR, MX and 2ME2 before irradiation enhances tumor radiosensitization and may improve therapeutic index for IUdR and 2ME2. Iran. J. Radiat. Res., 2010 7 (4): 211-216
Keywords: Hypoxia-inducible factor-1α, IUdR, radiosensitization, spheroid, methoxyamine, 2-methoxyestradiol.
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Type of Study: Original Research | Subject: Radiation Biology
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Neshasteh-Riz A, Babaloui S, Khoei S. Evaluation of combination effects of 2- methoxyestradiol and methoxyamine on IUdRinduced radiosensitization in glioma spheroids. Int J Radiat Res. 2010; 7 (4) :211-216
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Volume 7, Issue 4 (3-2010) Back to browse issues page
International Journal of Radiation Research
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