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:: Volume 10, Issue 3 And 4 (12-2012) ::
Int J Radiat Res 2012, 10(3 And 4): 177-181 Back to browse issues page
Comparison of radiosensitizing effect of Resveratrol on monolayer and spheroid culture of DU145 prostatic cell line
M.S. Nezamtaheri, S. Khoei, A.R. Nikoofar, B. Goliaei
, goliaei@ibb.ut.ac.ir
Abstract:   (8588 Views)
Background: Radiotherapy is an established therapeutic modality for prostate cancer. Resveratrol, a natural antioxidant, has been shown to inhibit carcinogenesis and to block the process of tumor initiation and progression. No data is available on the response of cellular spheroid to Reseveratol. In this study we have examined the effect of Resveratol on the radiation response of human prostate cell line DU145 in monolayer and spheroid cultures. Materials and Methods: Radiosensitivity was assessed using viability and colony formation assay. Apoptosis and necrosis were assessed using acridine orange/ ethidium bromide double staining. Results: The colony formation assay did not show any significant radio-sensitizing effect, but apoptosis assay showed significant radio-sensitizing effect of Resveratol on DU145 cells grown as monolayer. In the spheroid cells the results of apoptosis test were not significant and corresponded closely to the result of survival curve. Conclusion: While Resveratol could sensitize DU145 cells in monolayer to ionizing radiation, it did not have any effect on sensitivity of cells cultured in spheroid cultures. Iran. J. Radiat. Res., 2012 10(3‐4): 177‐181
Keywords: Resveratrol, X-ray irradiation, multicellular spheroid, radioresistance, apoptosis.
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Type of Study: Original Research | Subject: Radiation Biology
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Nezamtaheri M, Khoei S, Nikoofar A, Goliaei B. Comparison of radiosensitizing effect of Resveratrol on monolayer and spheroid culture of DU145 prostatic cell line. Int J Radiat Res. 2012; 10 (3 and 4) :177-181
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Volume 10, Issue 3 And 4 (12-2012) Back to browse issues page
International Journal of Radiation Research
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