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Analysis of microRNAs expression changes in human breast cancer cell lines following exposure to ionizing radiation
S. Dehghan Kouhestani , M. Forouzandeh Dr., M. Aghili
Abstract:   (70 Views)
Background: The expression alterations of specific miRNAs were analysed in response to gamma-irradiation in two breast cancer cell lines MCF-7 and MDA-MB-231 with low and high BCSCs content to design probable strategies for sensitizing breast cancer stem-like cells MDA-MB-231 to radiation. Materials and Methods: The expression levels of hsa-miR-34a, hsa-let-7i and hsa-miR-21 were assessed by QRT-PCR in MCF-7 and MDA-MB-231 breast cancer cells at three time points after gamma irradiation using three different doses. Results: The results showed that the over-expression of hsa-miR-34a in MCF-7 cells was much more significant than MDA-MB-231 cells. Furthermore, there was a considerable over-expression of hsa-miR-21 in MDA-MB-231 cells following exposure to ionizing radiation (IR). The hsa-let-7i expression changes were different, depending on radiation dose, time post irradiation and cell type. Conclusion: According to the results, we may be able to sensitize stem-like MDA-MB-231 breast cancer cells to radiation by increasing miR-34a and decreasing miR-21 expression levels simultaneously.
Keywords: miR-34a, miR-21, let-7i, ionizing radiation, breast cancer.
Full-Text [PDF 740 kb]   (9 Downloads)    
Type of Study: Case Report | Subject: Radiation Biology
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International Journal of Radiation Research
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