|
Analysis of microRNAs expression changes in human breast cancer cell lines following exposure to ionizing radiation
|
S. Dehghan Kouhestani   , M. Forouzandeh , M. Aghili |
| Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran |
|
|
Abstract: (4617 Views) |
| Background: The expression alterations of specific miRNAs were analysed in response to gamma-irradiation in two breast cancer cell lines MCF-7 and MDA-MB-231 with low and high BCSCs content to design probable strategies for sensitizing breast cancer stem-like cells MDA-MB-231 to radiation. Materials and Methods: The expression levels of hsa-miR-34a, hsa-let-7i and hsa-miR-21 were assessed by QRT-PCR in MCF-7 and MDA-MB-231 breast cancer cells at three time points after gamma irradiation using three different doses. Results: The results showed that the over-expression of hsa-miR-34a in MCF-7 cells was much more significant than MDA-MB-231 cells. Furthermore, there was a considerable over-expression of hsa-miR-21 in MDA-MB-231 cells following exposure to ionizing radiation (IR). The hsa-let-7i expression changes were different, depending on radiation dose, time post irradiation and cell type. Conclusion: According to the results, we may be able to sensitize stem-like MDA-MB-231 breast cancer cells to radiation by increasing miR-34a and decreasing miR-21 expression levels simultaneously. |
|
| Keywords: miR-34a, miR-21, let-7i, ionizing radiation, breast cancer. |
|
|
Full-Text [PDF 1723 kb]
(1633 Downloads)
|
|
Type of Study: Case Report |
Subject:
Radiation Biology
|
|
|
|
|
|
|
| Add your comments about this article |
|
|
|
