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The role of dual specificity phosphatase 1 in modulating hepatitis B virus-driven liver cancer progression and radiation response
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X. Lai   , G. Gao , L. Lin |
| Department of Liver Disease, Ningbo No.2 Hospital, Ningbo, Zhejiang Province, China , 15988699773@163.com |
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Abstract: (38 Views) |
Background: Dual-specificity phosphatase 1 (DUSP1) plays a critical role in cellular stress responses, but its function in hepatitis B virus (HBV)-associated hepatocellular carcinoma (LCC) and radiation therapy remains unclear. This study investigates the effects of DUSP1 on HBV replication, tumor progression, and radiation response in LIC. Materials and Methods: Fifteen HBV-LIC patients and 10 radiation-treated liver tumor patients (20–50 Gy) were included. HepG2.2.15 cells were used to establish DUSP1-overexpressing and control groups. DUSP1 expression was assessed via Western blot and quantitative polymerase chain reaction (qPCR). HBV replication markers (HBsAg, HBeAg) were measured by enzyme-linked immunosorbent assay (ELISA). Cell proliferation cell counting Kit-8 (CCK-8), migration/invasion (Transwell), and miR-21 expression were evaluated. Statistical analysis was performed using SPSS 22.0. Results: DUSP1 was significantly downregulated in LIC tissues (P < 0.001) and further suppressed in irradiated patients (P = 0.002). DUSP1 overexpression reduced HBV replication (HBsAg: P = 0.008; HBeAg: P = 0.008) and enhanced radiosensitivity. Overexpression inhibited proliferation, migration, and invasion (P < 0.001) and attenuated radiation-induced miR-21 upregulation (P = 0.004). Conclusion: DUSP1 suppresses HBV replication and tumor progression while enhancing radiation response in LIC, suggesting its potential as a therapeutic target for HBV-associated hepatocellular carcinoma. |
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| Keywords: pecificity phosphatases, hepatitis B virus, hepatocellular carcinoma, radiotherapy, microRNA-21, signal transduction. |
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Full-Text [PDF 813 kb]
(6 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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