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Reduction of cat allergen levels based on anti-Fel d1 andanti-Fel d4 IgY after radiotherapy
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W. Chai   , S. Han , M. Deng , J. Ma , Y. Li , Q. Shi , W. Zhang , Y. Xu , M. Dong , Z. Dai |
| School of Pharmacy, Qingdao University, Haoyuan Medical Education Comprehensive Building, Fushan Campus, Qingdao City, China , Mxdong64@qdu.edu.cn |
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Abstract: (12 Views) |
Background: Fel d 1 and Fel d 4 are the major feline allergens responsible for allergic responses in sensitized individuals. Immunoglobulin Y (IgY) antibodies can neutralize allergens, while low-dose radiotherapy may transiently alter glandular secretion. To evaluate the combined effect of anti-Fel d 1 and anti-Fel d 4 IgY and controlled low-dose radiotherapy on reducing allergen levels in cats. Materials and Methods: Cats received localized low-dose radiotherapy (2 Gy delivered in two fractions) followed by oral supplementation with purified and lyophilized anti-Fel d 1 and anti-Fel d 4 IgY. Allergen concentrations in saliva and hair were measured weekly over six weeks using ELISA. IgY purity, titer stability, and neutralizing activity were assessed in-vitro. Results: IgY purity ranged from 80–90% and titers remained stable after lyophilization. Radiotherapy alone produced a transient 10–15% reduction in salivary allergen secretion. With IgY administration, salivary Fel d 1 decreased from 4.50 μg/mL to 0.28 μg/mL (−94%) and hair Fel d 1 from 1.30 μg/mL to 0.15 μg/mL (−88%) by week six. Salivary Fel d 4 decreased from 12.0 μg/mL to 1.70 μg/mL (−86%). No radiation-related adverse effects were observed. Conclusion: Low-dose radiotherapy produced a short-term decrease in allergen secretion, while IgY supplementation resulted in a sustained and substantial reduction of Fel d 1 and Fel d 4 levels. This combined approach may represent a practical strategy for reducing environmental cat allergen exposure. |
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| Keywords: Fel d 1, Fel d 4, immunoglobulins, radiotherapy, cat allergens, hypersensitivity, enzyme-linked immunosorbent assay. |
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Full-Text [PDF 956 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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