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Effects of ginsenoside Rg3 on chemoradiosensitivity to paclitaxel in breast cancer cells and its correlation with autophagy-related genes
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S. Chen   , Y. Liu , H. Bao , E. Zhao , X. Qiao , M. Gu , J. Zhou , J. Zhang |
| , zhangjingyan1982@126.com |
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Abstract: (8 Views) |
Background: Resistance to paclitaxel (PTX) remains is a challenge in treatment of breast cancer, which is often mediated by multidrug resistance proteins and dysregulated autophagy. Ginsenoside Rg3 has been reported to enhance chemosensitivity in several malignancies, but its role in PTX resistance and underlying mechanisms in breast cancer remain unclear. Materials and Methods: In this study Human breast cancer MCF-7 cells treated with PTX, Rg3, or both. Cell viability was assessed by MTT assay, apoptosis by Annexin V/PI flow cytometry, and drug efflux by rhodamine-123 retention. Autophagy was evaluated using transmission electron microscopy, monodansylcadaverine and acridine orange staining, and Western blot analysis of autophagy-related proteins. Expression of MDR1/P-glycoprotein and Wnt/β-catenin signaling components was also analyzed. Results: Rg3 significantly enhanced PTX-induced growth inhibition and apoptosis in MCF-7 cells compared with PTX alone. Combination treatment increased intracellular rhodamine-123 accumulation, indicating reduced drug efflux. Autophagy activation was evidenced by increased LC3-II/LC3-I ratio and Beclin-1 expression, along with decreased p62 levels. Additionally, Rg3 combined with PTX downregulated MDR1/P-glycoprotein and suppressed key proteins of the Wnt/β-catenin signaling pathway. Correlation analysis revealed that autophagy markers were positively associated with apoptosis and negatively correlated with MDR1 expression. Conclusion: Ginsenoside Rg3 increases breast cancer cells chemosensitivity to paclitaxel, potentially through modulation of autophagy, inhibiting Wnt/β-catenin signaling, and suppressing multidrug resistance mechanisms. The findings of this study showed that Rg3 may represent a potential adjuvant for chemotherapy, while its role in modulating radiosensitivity needs further investigation in dedicated radiotherapy models. |
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| Keywords: Ginsenoside Rg3, paclitaxel, breast cancer, autophagy, drug resistance Wnt/β-catenin, radiosensitization, radiotherapy-related resistance. |
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Full-Text [PDF 1275 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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