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Patient-derived three-dimensional bioprinted models of low-grade glioma: A pilot feasibility study
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B. Hu   , S. Yang , Z. Zhang , Y. Liu , D. Jiang , J. Yang |
| CD BioEng & Therapy Co. Ltd, Ningbo, Zhejiang, China , yangj583@alumni.sysu.edu.cn |
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Abstract: (6 Views) |
Background: Patient-derived three-dimensional (3D) models may improve preclinical research of low-grade gliomas (LGGs), but the feasibility of bioprinting adult LGG tissue is poorly defined. Materials and Methods: Fresh surgical specimens from three patients with CNS WHO grade 2, IDH1-mutant gliomas were mechanically and enzymatically dissociated and incorporated into 7.5% or 8% gelatin methacryloyl bioinks. Constructs personalized to the patient were generated by extrusion bioprinting and cultured under physiological hypoxia for 35 days. Serial bright-field microscopy was used to assess morphological development and structural integrity. Intracellular ATP was measured longitudinally as an indirect marker of viable, metabolically active cells. Results: All three samples were successfully bioprinted into constructs. In the model from patient 1, tumor cells aggregated gradually during early culture and formed compact spheroid- or organoid-like structures by day 6–9. The printed architecture remained visible up to day 35 without apparent macroscopic collapse or total disintegration. In both GelMA formulations, the ATP-associated chemiluminescence in the Patient 1-derived model was not diminished as compared to the baseline at day 3 and remained high throughout the observation period, indicating persistent metabolic viability. The available data did not allow for a definitive comparison between the two concentrations of GelMA, nor for an evaluation of reproducibility across all patients. Conclusion: Patient-derived LGG cells can be encapsulated in 3D bioprinted GelMA constructs and cultured in-vitro for up to 35 days. These findings support technical feasibility and warrant validation in larger cohorts with molecular-fidelity and drug-response analyses. |
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| Keywords: Low-grade glioma, patient-derived organoid, 3D bioprinting, in-vitro model, tumor microenvironment. |
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Full-Text [PDF 1200 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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