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:: Volume 16, Issue 3 (7-2018) ::
Int J Radiat Res 2018, 16(3): 383-388 Back to browse issues page
Analysis of microRNAs expression changes in human breast cancer cell lines following exposure to ionizing radiation
S. Dehghan Kouhestani , M. Forouzandeh, M. Aghili
Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract:   (3070 Views)
Background: The expression alterations of specific miRNAs were analysed in response to gamma-irradiation in two breast cancer cell lines MCF-7 and MDA-MB-231 with low and high BCSCs content to design probable strategies for sensitizing breast cancer stem-like cells MDA-MB-231 to radiation. Materials and Methods: The expression levels of hsa-miR-34a, hsa-let-7i and hsa-miR-21 were assessed by QRT-PCR in MCF-7 and MDA-MB-231 breast cancer cells at three time points after gamma irradiation using three different doses. Results: The results showed that the over-expression of hsa-miR-34a in MCF-7 cells was much more significant than MDA-MB-231 cells. Furthermore, there was a considerable over-expression of hsa-miR-21 in MDA-MB-231 cells following exposure to ionizing radiation (IR). The hsa-let-7i expression changes were different, depending on radiation dose, time post irradiation and cell type. Conclusion: According to the results, we may be able to sensitize stem-like MDA-MB-231 breast cancer cells to radiation by increasing miR-34a and decreasing miR-21 expression levels simultaneously.
Keywords: miR-34a, miR-21, let-7i, ionizing radiation, breast cancer.
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Type of Study: Case Report | Subject: Radiation Biology
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Dehghan Kouhestani S, Forouzandeh M, Aghili M. Analysis of microRNAs expression changes in human breast cancer cell lines following exposure to ionizing radiation . Int J Radiat Res. 2018; 16 (3) :383-388
URL: http://ijrr.com/article-1-2286-en.html

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Volume 16, Issue 3 (7-2018) Back to browse issues page
International Journal of Radiation Research
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