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:: Volume 20, Issue 4 (10-2022) ::
Int J Radiat Res 2022, 20(4): 829-837 Back to browse issues page
Choline glycerophosphate and silymarin modulate brain and intestinal injuries in rats exposed to gamma-radiation
N. Abdel-Aziz , A.A. Elkady , E.M. Elgazzar
Health Radiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), P.O. Box 29, Nasr City, Cairo, Egypt , elkadyah13@gmail.com
Abstract:   (825 Views)
Background: the aim of this study was to investigate the role of choline glycerophosphate (GPC) either alone or combined with silymarin in modulating whole body gamma irradiation-induced brain and intestinal injuries in rats. Materials and Methods: Rats were irradiated with 7Gy then subjected to GPC and/ or silymarin for two weeks. At the end of the experiment, the animals were sacrificed then, brain and intestine samples were removed for biochemical, molecular and histopathological examinations. Results: it has been detected that GPC alone or combined with silymarin ameliorated the adverse effects of radiation as revealed by the inhibition of oxidative stress, apoptotic and inflammatory markers [Malondialdehyde (MDA), Caspase-3 Tumors necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nuclear factor kappa-B (NF-kB)]. However, Total antioxidant capacity (TAC), anti-inflammatory marker, Interleukin-10 (IL-10) and inhibitor of Nuclear factor kappa-Ba (IkBa) mRNA were increased. This was also accompanied by a significant increase in the Acetylcholine (ACh) level, Choline Acetyltransferase (ChAT) activity and Alpha-7 nicotinic receptor (α7nAChR), mRNA-expression and a significant decrease in the activity of Acetylcholine esterase (AChE) as compared with the corresponding values of the irradiated group. Moreover, a reduction in the tissue lesions was observed in brain and intestinal tissues. Conclusion: Choline glycerolphosphate and silymarin exhibited modulating effect against detrimental effects of gamma-radiation via cholinergic anti-inflammatory pathway.
 
 
Keywords: γ-radiation, choline glycerophosphate, silymarin, brain and intestinal injury.
Full-Text [PDF 1334 kb]   (514 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
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Abdel-Aziz N, Elkady A, Elgazzar E. Choline glycerophosphate and silymarin modulate brain and intestinal injuries in rats exposed to gamma-radiation. Int J Radiat Res 2022; 20 (4) :829-837
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Volume 20, Issue 4 (10-2022) Back to browse issues page
International Journal of Radiation Research
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