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:: Volume 21, Issue 4 (10-2023) ::
Int J Radiat Res 2023, 21(4): 609-614 Back to browse issues page
Fractionated radiation exposure enhances the DNA repair capacity to acquire radioresistance in HCT8 human colorectal cancer cells
K. Huang , M. Omura , C. Yan , L. Abdelghany , X. Zhang , T-S. Li
Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan , litaoshe@nagasaki-u.ac.jp
Abstract:   (1082 Views)
Background: Fractionated radiotherapy is widely used for cancer treatment because of its advantages in the preservation of normal tissues; however, it may amplify the radioresistance of cancer cells. In this study, we aimed to understand whether and how fractionated radiation exposure induces radioresistance. Materials and Methods: HCT8 human colorectal cancer cells received a total X-ray dose of 5 Gy in either a single treatment (5 Gy administered once) or via fractionated exposure (1 Gy/day treatment for 5 consecutive days). We then examined the radioresistance of cancer cells exposed an additional 2 Gy X-ray by clonogenic assay and Western blot analysis. Results: Cells receiving fractionated exposure showed significantly greater proliferation and clonogenicity than those that received a single dose. Compared with the levels in the intact cells without radiation exposure, the expression levels of γH2AX, phospho-ATM and PARP were significantly enhanced only in the cells exposed to fractionated radiation. In contrast, the expression of cyclin D1 and cyclin E1 was enhanced only in the cells that received a single dose. In addition, the expression of SOD1 and SOD2 was slightly increased in the cells that received either the fractionated exposure or single exposure treatment. Conclusions: Fractionated radiation exposure facilitates radioresistance in HCT8 human colorectal cancer cells predominantly by enhancing their DNA repair capacity.
Keywords: Fractionated radiation exposure, DNA repair, Acquired radioresistance.
Full-Text [PDF 1034 kb]   (774 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
References
1. Delaney G, Jacob S, Featherstone C, Barton M (2005) The role of radiotherapy in cancer treatment: estimating optimal utilization from a review of evidence-based clinical guidelines. Cancer, 104(6): 1129-1137.
2. Maverakis E, Cornelius LA, Bowen GM, et al. (2015) Metastatic melanoma - a review of current and future treatment options. Acta Derm Venereol, 95(5): 516-524.
3. Balmukhanov SB, Yefimov ML, Kleinbock TS (1967) Acquired radioresistance of tumour cells. Nature. 216(5116): 709-711.
4. West CM, Davidson SE, Elyan SA, et al. (1998) The intrinsic radiosensitivity of normal and tumour cells. Int J Radiat Biol, 73(4): 409-413.
5. Baumann M, Krause M, Overgaard J, et al. (2016) Radiation oncology in the era of precision medicine. Nat Rev Cancer, 16(4): 234-249.
6. Pfeffer CM and Singh ATK (2018) Apoptosis: A Target for Anticancer Therapy. Int J Mol Sci, 19(2): 448.
7. Schulz A, Meyer F, Dubrovska A, Borgmann K (2019) Cancer stem cells and radioresistance: DNA Repair and Beyond. Cancers (Basel), 11(6): 862.
8. Venere M, Hamerlik P, Wu Q, et al. (2014) Therapeutic targeting of constitutive PARP activation compromises stem cell phenotype and survival of glioblastoma-initiating cells. Cell Death Differ, 21(2): 258-269.
9. Wilson WR and Hay MP (2011) Targeting hypoxia in cancer therapy. Nat Rev Cancer, 11(6): 393-410.
10. Citrin D E (2017) Recent Developments in Radiotherapy. N Engl J Med, 377(11): 1065-1075.
11. Goschzik T, Schwalbe EC, Hicks D, et al. (2018) Prognostic effect of whole chromosomal aberration signatures in standard-risk, non-WNT/non-SHH medulloblastoma: a retrospective, molecular analysis of the HIT-SIOP PNET 4 trial. Lancet Oncol, 19(12): 1602-1616.
12. De Bari B, Daidone A, Alongi F (2015) Is high dose rate brachytherapy reliable and effective treatment for prostate cancer patients? A review of the literature. Crit Rev Oncol Hematol, 94(3): 360-370.
13. Lannering B, Rutkowski S, Doz F, et al. (2012) Hyperfractionated versus conventional radiotherapy followed by chemotherapy in standard-risk medulloblastoma: results from the randomized multicenter HIT-SIOP PNET 4 trial. J Clin Oncol, 30(26): 3187-3193.
14. Sato K, Shimokawa T, Imai T (2019) Difference in acquired radioresistance induction between repeated photon and particle irradiation. Front Oncol, 9: 1213.
15. Osuka S, Zhu D, Zhang Z, et al. (2021) N-cadherin upregulation mediates adaptive radioresistance in glioblastoma. J Clin Invest, 131(6): e136098.
16. Zhang X, Fang B, Mohan R, Chang JY (2012) Coxsackie-adenovirus receptor as a novel marker of stem cells in treatment-resistant non-small cell lung cancer. Radiother Oncol, 105(2): 250-257.
17. Tompkins WA, Watrach AM, Schmale JD, et al. (1974) Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J Natl Cancer Inst, 52(4): 1101-1110.
18. Shimura T, Kakuda S, Ochiai Y, et al. (2011) Targeting the AKT/GSK3β/cyclin D1/Cdk4 survival signaling pathway for eradication of tumor radioresistance acquired by fractionated radiotherapy. Int J Radiat Oncol Biol Phys, 80(2): 540-548.
19. Li X F, Ma L, Lu J, et al. (2013) Effect of ionizing radiation on transcription of colorectal cancer MDR1 gene of HCT-8 cells. Asian Pac J Trop Med, 6(5): 407-409.
20. Ji D, Zhan T, Li M, et al. (2018) Enhancement of Sensitivity to Chemo/Radiation Therapy by Using miR-15b against DCLK1 in Colorectal Cancer. Stem Cell Reports, 11(6): 1506-1522.
21. Guichard M, Dertinger H, Malaise EP (1983) Radiosensitivity of four human tumor xenografts. Influence of hypoxia and cell-cell contact. Radiat Res, 95(3): 602-609.
22. Rezacova M, Rudolfova G, Tichy A, et al. (2011) Accumulation of DNA damage and cell death after fractionated irradiation. Radiat Res, 175(6): 708-718.
23. Rubner Y, Muth C, Strnad A, et al. (2014) Fractionated radiotherapy is the main stimulus for the induction of cell death and of Hsp70 release of p53 mutated glioblastoma cell lines. Radiat Oncol, 9(1): 89.
24. Singh B, Patwardhan RS, Jayakumar S, et al. (2020) Oxidative stress associated metabolic adaptations regulate radioresistance in human lung cancer cells. J Photochem Photobiol B, 213: 112080.
25. Kunnumakkara AB, Diagaradjane P, Guha S, et al. (2008) Curcumin sensitizes human colorectal cancer xenografts in nude mice to gamma-radiation by targeting nuclear factor-kappaB-regulated gene products. Clin Cancer Res, 14(7): 2128-2136.
26. van den Berg J, Castricum KCM, Meel MH, et al. (2020) Development of transient radioresistance during fractionated irradiation in vitro. Radiother Oncol, 148: 107-114.
27. Schaue D and McBride WH (2015) Opportunities and challenges of radiotherapy for treating cancer. Nat Rev Clin Oncol, 12(9): 527-540.
28. Wong WK, Guerra Liberal FDC, McMahon SJ (2022) DNA Repair inhibitors potentiate fractionated radiotherapy more than single-dose radiotherapy in breast cancer cells. Cancers (Basel), 14(15): 3794.
29. Krueger SA, Collis SJ, Joiner MC, et al. (2007) Transition in survival from low-dose hyper-radiosensitivity to increased radioresistance is independent of activation of ATM Ser1981 activity. Int J Radiat Oncol Biol Phys, 69(4): 1262-1271.
30. Tsuchida E, Kaida A, Pratama E, et al. (2015) Effect of X-irradiation at different stages in the cell cycle on individual cell-based kinetics in an asynchronous cell population. PLoS One, 10(6): e0128090.
31. Gerdes J, Lemke H, Baisch H, et al. 1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol, 133(4): 1710-1715.
32. Borges HL, Linden R, Wang JY (2008) DNA damage-induced cell death: lessons from the central nervous system. Cell Res, 18(1): 17-26.
33. Tchakarska G and Sola B (2020) The double dealing of cyclin D1. Cell Cycle, 19(2): 163-178.
34. Shimura T, Ochiai Y, Noma N, et al. (2013) Cyclin D1 overexpression perturbs DNA replication and induces replication-associated DNA double-strand breaks in acquired radioresistant cells. Cell Cycle, 12(5): 773-782.
35. Aruga T, Ando K, Iizuka M, et al. (1995) Radiosensitivity and cell cycle redistribution of cultured human tumour cells during fractionated daily 2-Gy irradiations. Int J Radiat Biol, 67(1): 65-70.
36. Lee SY, Jeong EK, Ju MK, et al. (2017) Induction of metastasis, cancer stem cell phenotype, and oncogenic metabolism in cancer cells by ionizing radiation. Mol Cancer, 16(1): 10.
37. Jiang H, Wang H, De Ridder M (2018) Targeting antioxidant enzymes as a radiosensitizing strategy. Cancer Lett, 438: 154-164.
38. Brahme A (2011) Accurate description of the cell survival and biological effect at low and high doses and LET's. J Radiat Res, 52(4): 389-407.
39. Hahnfeldt P and Hlatky L (1996) Resensitization due to redistribution of cells in the phases of the cell cycle during arbitrary radiation protocols. Radiat Res, 145(2): 134-143.
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Huang K, Omura M, Yan C, Abdelghany L, Zhang X, Li T. Fractionated radiation exposure enhances the DNA repair capacity to acquire radioresistance in HCT8 human colorectal cancer cells. Int J Radiat Res 2023; 21 (4) :609-614
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Volume 21, Issue 4 (10-2023) Back to browse issues page
International Journal of Radiation Research
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